|
|
|
| Serum metabolism characteristics and biomarker of joint exposure to nonylphenol and octylphenol |
| LIU Chun-hong1,2, WANG Wei-hua1, SUN Yuan-ming1,2, LIN Feng3, HUANG Shao-wen1 |
1. Food College, South China Agricultural University, Guangzhou 510642, China;
2. Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou 510642, China;
3. Guangdong Inspection and Quarantine Technology Center Food Testing Lab, Guangzhou 510623, China |
|
|
|
|
Abstract The metabonomics method was employed to investigate the changes in serum metabolic components of Sprague Dawley rats after combined exposure to nonylphenol (NP) and octylphenol (OP). The male rats were randomly divided into control group, low-dose combined exposure group (25mg/kg NP+25mg/kg OP) and high-dose combined exposure group (75mg/kg NP+75mg/kg OP). The rats were treated by gavage once a day for 7 days, and then killed. The metabolic fingerprinting of rat serum was obtained by ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC/TOF-MS). Dose-response toxicity was observed based on the principle components analysis (PCA). Meanwhile, the potential biomarkers were screened out according to t-test. The results showed that there was a significant difference in the metabolic profile among the control group, high and low dose groups, and with the increase of exposure dose, the toxicity was enhanced, which showed a dose-effect relationship. Meanwhile, the contents of taurocholic acid and 1-Palmitoyllysophosphatidylcholine increased while the contents of tyrosine, uridine-5'-monophosphate and sulfatide decreased under combined exposure to NP and OP. It is suggested that NP and OP exposure may have adverse effects on the endocrine system, cardiovascular system, nervous system, nucleotide synthesis, sugar and phospholipid metabolism.
|
|
Received: 10 April 2016
|
|
|
|
|
|
| [1] |
Ying G G, Williams B, Kookana R. Environmental fate of alkylphenols and alkylphenol ethoxylates-a review[J]. Environment International, 2000,28:215-226.
|
| [2] |
Janicki T, Krupiński M, Dlugoński J. Degradation and toxicity reduction of the endocrine disruptors nonylphenol, 4-tertoctylphenol and 4-cumylphenol by the non-ligninolytic fungus Umbelopsis isabellina[J]. Bioresource Technology, 2016,200:223-229.
|
| [3] |
刘媛,张彩香,廖小平,等.太原小店污灌区土壤壬基酚的分布特征[J]. 中国环境科学, 2015,35(1):165-170.
|
| [4] |
柳春红,王伟华,褚玥等.深圳居民膳食壬基酚和辛基酚暴露的风险评估[J]. 中国环境科学, 2013,33(7):1316-1322.
|
| [5] |
谭睿婕,李宗圣,刘瑞霞,等.典型内分泌干扰物在天然水环境中的污染水平[J]. 安徽农业科技, 2015,43(23):167-169,288.
|
| [6] |
康海宁,程巧,涂小珂,等.珠江八大河口表层沉积物中典型环境内分泌干扰物的分布特征[J]. 食品安全质量检测学报, 2014,5(11):3386-3393.
|
| [7] |
沈钢,张祖麟,余刚,等.夏季海河与渤海湾中壬基酚和辛基酚污染的状况[J]. 中国环境科学, 2005,25(6):733-736.
|
| [8] |
Soares A, Guieysse B, Jefferson B, et al. Nonylphenol in the environment:a critical review on occurrence, fate, toxicity and treatment in wastewaters[J]. Environment International, 2008,34:1033-1049.
|
| [9] |
Lee H R, Choi K C. 4-tert-Octylphenol stimulates the expression of cathepsins in human breast cancer cells and xenografted breast tumors of a mouse model via an estrogen receptor-mediated signaling pathway[J]. Toxicology, 2013,304:13-20.
|
| [10] |
Inadera H. The immune system as a target for environmental chemicals:xenoestrogens and other compounds[J]. Toxicology Letters, 2006,164:191-206.
|
| [11] |
许国旺.代谢组学方法与应用[M]. 北京:科学出版社, 2008.
|
| [12] |
Athersuch T. Metabolome analyses in exposome studies:Profiling methods for a vast chemical space[J]. Archives of Biochemistry and Biophysics, 2016,589:177-186.
|
| [13] |
McKelvie J R, Wolfe D M, Celejewski M A, et al. Metabolic responses of Eisenia fetida after sub-lethal exposure to organic contaminants with different toxic modes of action[J]. Environmental Pollution. 2011,159(12):3620-3626.
|
| [14] |
Lee S H, Woo H M, Jung B H, et al. Metabolomic approach to evaluate the toxicological effects of nonylphenol with rat urine[J]. Analytical Chemistry, 2007,79:6102-6110.
|
| [15] |
王伟华,柳春红,孙远明,等.大鼠尿液中壬基酚的代谢轮廓[J]. 高等学校化学学报, 2011,32(10):2280-2285.
|
| [16] |
柳春红,王伟华,孙远明,等.壬基酚和辛基酚联合染毒的尿液代谢组学研究[J]. 分析化学, 2012,40(1):113-118.
|
| [17] |
杨绍明,林汉枫,江秀明,等.血液中苯丙氨酸的测定方法研究进展[J]. 分析测试学报, 2003,22(6):113-117.
|
| [18] |
Cansev M, Watkins C J, van der Beek E M, et al. Oral uridine-5'-monophosphate (UMP) increases brain CDP-choline levels in gerbils[J]. Brain Research, 2005,1058(1/2):101-108.
|
| [19] |
Krylova I B, Kachaeva E V, Rodionova O M, et al. The cardioprotective effect of uridine and uridine-5'-monophosphate:the role of the mitochondrial ATP-dependent potassium channel[J]. Experimental Gerontology, 2006,41(7):697-703.
|
| [20] |
Park J W, Colloids S B. Sulfatide incorporation effect on mechanical properties of vesicles[J]. Colloids and Surfaces B:Biointerfaces, 2010,80(1):59-62.
|
| [21] |
Han X. Lipid alterations in the earliest clinically recognizable stage of Alzheimer's disease:Implication of the role of lipids in the pathogenesis of Alzheimer's disease[J]. Current Alzheimer Research, 2005,2(1):65-77.
|
| [22] |
Osterbye T, Jørgensen K H, Fredman P, et al. Sulfatide promotes the folding of proinsulin, preserves insulin crystals, and mediates its monomerization[J]. Glycobiology, 2001,11(6):473-479.
|
| [23] |
Kyogashima M, Sakai T, Onaya J, et al. Roles of galactose and sulfate residues in sulfatides for their antagonistic functions in the blood coagulation system[J]. Glycoconjugate Journal, 2001,18(3):245-251.
|
| [24] |
刘欣媛,李培锋.牛磺胆酸研究进展[J]. 包头医学院学报, 2010,26(4):134-135.
|
| [25] |
宋丹军,潘家琪,李鹏旭,等.溶血磷脂酰胆碱在肝脏疾病中的研究进展[J]. 中国药理学通报, 2014,30(12):1642-1646.
|
| [26] |
Ding W G, Toyoda F, Ueyama H, et al. Lysophosphatidylcholine enhances I(Ks) currents in cardiac myocytes through activation of G protein, PKC and Rho signaling pathways[J]. Journal of Molecular and Cellular Cardiology, 2011,50(1):58-65.
|
| [27] |
Kim S C, Kim M K, Kim Y H, et al. Differential levels of L-homocysteic acid and lysophosphatidylcholine (16:0) in sera of patients with ovarian cancer[J]. Oncology Letters, 2014,8(2):566-574.
|
|
|
|
