藻源性颗粒有机物对磷饥饿微囊藻磷富集与生长的影响

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Abstract The phytoplankton-derived particulate organic matter (POM) in Lake Taihu was taken as the breakthrough point in this study which referenced phosphorus in the orthophosphate (K₂HPO₄), polyphosphate (Na₅P₃O₁₀), orthophosphate monoester (G-6-P) and the orthophosphate diester (lecithin). The effects of phosphorus in different forms on the growth and phosphorus enrichment of phosphorus-deficiency M. aeruginosa was studied. Results showed that the phosphorus-deficiency M. aeruginosa with density of (1.9±0.1)×10⁶cells/mL cannot directly use Na₅P₃O₁₀ and lecithin whose short-term enrichment were 2.034mg/L, 1.030mg/L. It was significantly higher than the 0.491mg/L of K₂HPO₄, the 0.034mg/L of G-6-P and the 0.573mg/L of phytoplankton-derived particulate organic matter (POM). Maybe these forms of P cannot enter the cells and were accumulated between the cell membrane and cell wall. M. aeruginosa can use the phytoplankton-derived POM rapidly by releasing alkaline phosphatase enzymes. The growth rate of M. aeruginosa with phytoplankton-derived POM was 0.148d^-1 and was slightly lower than the 0.156d^-1of K₂HPO₄, but it was much higher than the rate of other groups. The utilization rate of phytoplankton-derived POM in the end of the experiment was 57.8% which was higher than the 32.5% of K₂HPO₄ and the 24.4% of lecithin. The composition of phosphorus in phytoplankton-derived POM were mainly orthophosphate and monoester by using liquid-phase NMR analysis. And we verified the effects of it on the growth and phosphorus enrichment of phosphorus-deficiency M. aeruginosa by calculation. In conclusion, the phytoplankton-derived POM had a strong bioavailability, and was very important to the outbreak and persistence on algae bloom.

Key words： phytoplankton-derived POM phosphorus enrichment growth rate Microcystis aeruginosa

Received: 16 April 2016

PACS:

X524

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	Articles by authors
	HE Dong
	CHAO Jian-ying
	ZHANG Yi-min
	YANG Fei
	WANG Yu
	GUO Yan-ming

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HE Dong,CHAO Jian-ying,ZHANG Yi-min等. Effects of the phytoplankton-derived particulate organic matter on the growth and phosphorus enrichment of phosphorus-deficiency Microcystis aeruginosa[J]. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(12): 3777-3783.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2016/V36/I12/3777

[1]	Redfield A C.The influence of organisms on the composition of sea-water[J].The sea,1963:26-77.
[2]	余先旭,孙石,朱宝平,等.磷与湖泊富营养化[J].云南环境科学,2005,24(S1):36-38.
[3]	Wynne D,Kaplan B,Berman T.Phosphatase Activities in Lake Kinneret Phytoplankton[M].Springer New York,1991:220-226.
[4]	Mimura T,Reid R J,Smith F A.Control of phosphate transport across the plasma membrane of Chara corallina[J].Journal of Experimental Botany,1998,volume 49(318):13-19(17).
[5]	Kulaev I,Vagabov V,Kulakovskaya T.New aspects of inorganic polyphosphate metabolism and function[J].Journal of Bioscience&Bioengineering,1999,88(2):111-129.
[6]	Naoshi F,Ryuichi S,Norio S,et al.Nutrient-limited growth of Microcystis aeruginosa and Phomzidium tenue and competition under various N:P supply ratios and temperatures[J].Limnology&Oceanography,1997,42(2):250-256.
[7]	杨英利.藻类对苯胺和三唑磷的积累、降解和抗氧化酶反应[D].武汉:华中师范大学,2006.
[8]	武晓飞,李大鹏,汪明,等.反复扰动下加藻对不同形态磷相互转化的影响[J].中国环境科学,2015,35(4):1187-1196.
[9]	李爱民,王学东,杨英利,等.3种淡水藻类对三唑磷的富集行为研究[J].华中师范大学学报:自然科学版,2008,41(4):565-568.
[10]	沈宏,宋立荣,周培疆,等.有机磷农药对滇池微囊藻生长和摄磷效应的影响[J].水生生物学报,2007,31(6):863-868.
[11]	刘璐,闫浩,夏文彤,等.镉对铜绿微囊藻和斜生栅藻的毒性效应[J].中国环境科学,2014,34(2):478-484.
[12]	杨承虎,刘洋之,朱亚先,等.铜绿微囊藻胞内DOM光降解及其对芘结合能力的影响[J].中国环境科学,2016,36(6):1850-1858.
[13]	高光,朱广伟,秦伯强,等.太湖水体中碱性磷酸酶的活性及磷的矿化速率[J].中国科学,2005,35(z2):157-165.
[14]	陈宇炜,高锡云,秦伯强.西太湖北部夏季藻类种间关系的初步研究[J].湖泊科学,1998,(4):35-40.
[15]	张毅敏,王宇,杨飞,等.太湖不同生态型湖区悬浮颗粒磷空间分布和降解速率[J].中国环境科学,2016,36(7):2128-2138.
[16]	何东,张毅敏,杨飞,等.太湖藻源性颗粒物降解过程中营养盐转化及其生态效应[J].中国环境科学,2016,36(3):899-907.
[17]	屠清瑛,金相灿.湖泊富营养化调查规范[M].2版.北京:中国环境科学出版社,1990.
[18]	黄清辉,王东红,马梅,等.沉积物和土壤中磷的生物有效性评估新方法[J].环境科学,2005,26(2):206-208.
[19]	Turner B L,Mahieu N,Condron L M.Phosphorus 31 Nuclear Magnetic Resonance Spectral Assignments of Phosphorus Compounds in Soil NaOH-EDTA Extracts[J].Soil Science Society of America Journal,2003,67(2):497-510.
[20]	王晓钧,丁士明,蒋海青,等.湖泊磷的核磁共振(NMR)分析——1环境标准样品的^31P-NMR表征[J].湖泊科学,2008,20(1):45-50.
[21]	金相灿,郑朔方.有机磷和无机磷对铜绿微囊藻生长的影响及动力学分析[J].环境科学研究,2006,19(5):40-44.
[22]	Zhou P J,Lin J,Shen H,et al.Kinetic studies on the combined effects of lanthanum and cerium on the growth of Microcystis aeruginosa and their accumulation by M.aeruginosa[J].Bulletin of Environmental Contamination&Toxicology,2004,72(4):711-716.
[23]	Levine S N,Stainton M P,Schindler D W.A Radiotracer Study of Phosphorus Cycling in a Eutrophic Canadian Shield Lake,Lake 227,Northwestern Ontario[J].Canadian Journal of Fisheries&Aquatic Sciences,1986,43(2):366-378.
[24]	Berman T,Skyring G W.Phosphorus cyeling in aquatic microorganisms studied by phased uptake of 33P and 32P[J].Current Microbiology,1979,2(1):47-49.
[25]	姚波,席北斗,胡春明,等.缺磷胁迫后四尾栅藻在富磷环境中对磷的吸收动力学[J].环境科学研究,2010,23(4):420-425.
[26]	Bierman V J,Dolan D M.Modeling of Phytoplankton-Nutrient Dynamics in Saginaw Bay,Lake Huron[J].Journal of Great Lakes Research,1981,7(4):409-439.
[27]	黄邦钦,黄世玉.溶解态磷在海洋微藻碱性磷酸酶活力变化中的调控作用[J].海洋学报:中文版,1999,(1):55-60.
[28]	钱善勤,孔繁翔,史小丽,等.不同形态磷酸盐对铜绿微囊藻和蛋白核小球藻生长的影响[J].湖泊科学,2008,20(6):796-801.
[29]	Ahlgren J,Reitzel K,Tranvik L,et al.Degradation of organic phosphorus compounds in anoxic Baltic Sea sediments:A 31P nuclear magnetic resonance study[J].Limnology&Oceanography,2006,51(5):2341-2348.
[30]	Qian S,Kong F S,Xiaoli,Zhang M,et al.Interspecific Interaction between Microcystis aeruginosa and Chlorella pyrenoidosa in Different Phosphate Media[J].Journal of Freshwater Ecology,2008,23(4):635-642.
[31]	Shi X,Qian S,Kong F,et al.Differences in growth and alkaline phosphatase activity between Microcystis aeruginosa and Chlorella pyrenoidosa in response to media with different organic phosphorus[J].Journal of Limnology,2011,70(1):21-25.
[32]	史小丽,王凤平,蒋丽娟,等.扰动对外源性磷在模拟水生态系统中迁移的影响[J].中国环境科学,2002,22(6):537-541.
[33]	钱善勤,孔繁翔,张民,等.铜绿微囊藻和蛋白核小球藻对不同形态有机磷的利用及其生长[J].湖泊科学,2010,22(3):411-415.
[34]	杨柳燕,王勤,史小丽,等.铜绿微囊藻磷代谢过程研究[J].农业环境科学学报,2005,24(4):686-689.
[35]	张文强,单保庆.基于液相(31) P核磁共振(NMR)技术的巢湖沉积物中有机磷形态研究[J].环境科学学报,2013,33(7):1967-1973.
[36]	Watts E E,Dean P a W,Martin R R.31P Nuclear magnetic resonance study of sediment microbial phospholipids[J].Canadian Journal of Analytical Sciences&Spectroscopy,2002,47(5):127-133.
[37]	邹迪,肖琳,杨柳燕,等.不同形态磷源对铜绿微囊藻与附生假单胞菌磷代谢的影响[J].环境科学,2005,26(3):118-121.
[38]	黄世玉,黄邦钦.不同磷源对藻类生长及其生化组成的影响[J].应用海洋学学报,1997,(4):458-464.