生活垃圾填埋场对河流抗生素抗性基因的影响

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Abstract

Aquatic environment especially the river is an important reservoir of antibiotic resistance genes (ARGs). For the further insight into the ARGs in a river in the vicinity of a landfill, high-throughput qPCR technique was used to investigate the diversity and abundance of ARGs. The results showed that a total of 57ARGs was detected in control sample, while, 158ARGs was detected in the downstream river sample, in which 150ARGs in the downstream river were significantly enriched (P<0.05). A maximum enrichment of 2299-fold was detected in dfrA1, which conferred resistance to sulfonamide. The detection number of ARGs and the enrichment of ARGs were significantly increased along the river. The abundance of mobile genetic elements was significantly correlated with the abundance of ARGs. These results implied that landfill may lead to the dissemination and enrichment of ARGs in river.

Key words： landfill river antibiotic antibiotic resistance genes

Received: 22 March 2016

PACS:

X703.1

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	Articles by authors
	HUANG Fu-yi
	AN Xin-li
	LI Li
	OUYANG Wei-ying
	LI Hu
	SU Jian-qiang

Cite this article:

HUANG Fu-yi,AN Xin-li,LI Li等. High-throughput profiling of antibiotic resistance genes in river in the vicinity of a landfill[J]. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(1): 203-209.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2017/V37/I1/203

[1]	Pruden A,Pei R,Storteboom H,et al.Antibiotic resistance genes as emerging contaminants:studies in northern Colorado[J].Environmental Science&Technology,2006,40(23):7445-7450.
[2]	苏建强,黄福义,朱永官.环境抗生素抗性基因研究进展[J].生物多样性,2013,21(4):481-487.
[3]	Zhu Y G,Johnson T A,Su J Q,et al.Diverse and abundant antibiotic resistance genes in Chinese swine farms[J].Proceedings of the National Academy of Sciences,2013,110(9):3435-3440.
[4]	汤薪瑶,左剑恶,余忻,等.制药废水中头孢类抗生素残留检测方法及环境风险评估[J].中国环境科学,2014,34(9):2273-2278.
[5]	Ouyang W Y,Huang F Y,Zhao Y,et al.Increased levels of antibiotic resistance in urban stream of Jiulongjiang River,China[J].Applied Microbiology and Biotechnology,2015,99(13):5697-5707.
[6]	Pei R,Kim S C,Carlson K H,et al.Effect of river landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG)[J].Water Research,2006,40(12):2427-2435.
[7]	黄福义,李虎,韦蓓,等.长期施用猪粪水稻土抗生素抗性基因污染研究[J].环境科学,2014,35(10):3869-3873.
[8]	黄福义,李虎,安新丽,等.城市生活污水和生活垃圾渗滤液抗生素抗性基因污染的比较研究[J].环境科学,2016,37(10):3949-3954.
[9]	Xu L K,Ouyang W Y,Qian Y Y,et al.High-throughput profiling of antibiotic resistance genes in drinking water treatment plants and distribution systems[J].Environmental Pollution,2016,213:119-126.
[10]	Hoge C W,Gambel J M,Srijan A,et al.Trends in antibiotic resistance among diarrheal pathogens isolated in Thailand over 15years[J].Clinical Infectious Diseases,1998,26(2):341-345.
[11]	Weber D J,Raasch R,Rutala W A.Nosocomial infections in the ICU:the growing importance of antibiotic-resistant pathogens[J].CHEST Journal,1999,115(suppl_1):34S-41S.
[12]	Fischbach M A,Walsh C T.Antibiotics for emerging pathogens[J].Science,2009,325(5944):1089-1093.
[13]	Ahammad Z S,Sreekrishnan T,Hands C L,et al.Increased waterborne bla NDM-1resistance gene abundances associated with seasonal human pilgrimages to the Upper Ganges River[J].Environmental Science&Technology,2014,48(5):3014-3020.
[14]	Allen S E,Boerlin P,Janecko N,et al.Antimicrobial resistance in generic Escherichia coli isolates from wild small mammals living in swine farm,residential,landfill,and natural environments in southern Ontario,Canada[J].Applied and Environmental Microbiology,2011,77(3):882-888.
[15]	余建恒,赵淑贤,夏耿东,等.接人垃圾渗滤液对城市污水厂运行的影响与对策[J].中国给水排水,2010,(4):95-97.
[16]	CJJ17-2004生活垃圾卫生填埋技术规范[S].
[17]	Stokes H W,Nesbø C L,Holley M,et al.Class 1integrons potentially predating the association with Tn402-like transposition genes are present in a sediment microbial community[J].Journal of Bacteriology,2006,188(16):5722-5730.
[18]	Gillings M R,Gaze W H,Pruden A,et al.Using the class 1integron-integrase gene as a proxy for anthropogenic pollution[J].The ISME Journal,2015,9(6):1269-1279.
[19]	R Core Team (2016).R:A language and environment for statistical computing.R Foundation for Statistical Computing,Vienna,Austria.URL https://www.R-project.org/.
[20]	Looft T,Johnson T A,Allen H K,et al.In-feed antibiotic effects on the swine intestinal microbiome[J].Proceedings of the National Academy of Sciences,2012,109(5):1691-1696.
[21]	Schmittgen T D,Livak K J.Analyzing real-time PCR data by the comparative CT method[J].Nature Protocols,2008,3(6):1101-1108.
[22]	D'Costa V M,McGrann K M,Hughes D W,et al.Sampling the antibiotic resistome[J].Science,2006,311(5759):374-377.
[23]	张致平.β-内酰胺类抗生素研究的进展[J].中国抗生素杂志,2000,25(2):81-86.
[24]	杜德才,周书明,沈爱宗,等.医院抗菌药物使用强度分析[J].中华医院感染学杂志,2010,20(6):848-851.
[25]	Wu D,Huang Z,Yang K,et al.Relationships between antibiotics and antibiotic resistance gene levels in municipal solid waste leachates in Shanghai,China[J].Environmental Science&Technology,2015,49(7):4122-4128.
[26]	Ciric L,Mullany P,Roberts A P.Antibiotic and antiseptic resistance genes are linked on a novel mobile genetic element:Tn6087[J].Journal of Antimicrobial Chemotherapy,2011,66(10):2235-2239.
[27]	Cruz F,Davies J.Horizontal gene transfer and the origin of species:lessons from bacteria[J].Trends in Microbiology,2000,8(3):128-133.
[28]	Kurland C G,Canback B,Berg O G.Horizontal gene transfer:a critical view[J].Proceedings of the National Academy of Sciences,2003,100(17):9658-9662.