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Optimization for pyrene bacteria cometabolism degradation under the low temperature and high salt environment through response surface |
DIAO Shuo, WANG Hong-qi, WU Xiao-xiong, ZHAO Yi-cun |
College of Water Sciences, Beijing Normal University, Beijing 100875, China |
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Abstract In order to enhance pyrene bacteria cometabolism degradation rate under low temperature and high salt environment, under the circumstances of learned optimal pH, initial concentration of pyrene, shaking rates of rocking bed, inoculating dosage, Plackett-Burman Design was conducted and glucose, salicylic acid and phenanthrene were identified as main factors. These factors were approached to optimal region by steepest ascent design. Then optimal degradation condition(glucose, 225.83mg/L; salicylic acid, 112.10mg/L; phenanthrene, 198.06mg/L) was established by Box-Behnken design and reponse surface analysis. Degradation rate under the optimal consition was 50.69% after 10days, enhanced 23.14% compare to the condition of without cometabolism degradation substrate. Experimental results also showed that the method of optimization for bacteria cometabolism degradation is reasonable and feadible.
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Received: 21 March 2016
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