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| Analysis of extracellular polymeric substances and bacterial community in mixed cultures for Cr(VI) reduction |
| ZHANG En-hua1,2, DAI You-fen2,3, XIAO Yong2, CHEN Bi-lian3, YANG Zhao-hui1, ZHAO Feng2 |
1. Key Laboratory of Environmental Biology and Pollution Control, College of Environmental Science and Engineering, Hunan University, Changsha 410082, China;
2. Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;
3. College of Life Sciences, Fujian Normal University, Fuzhou 350108, China |
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Abstract To better understand the mechanisms of Cr (VI) reduction by mixed microbial communities, Cr (VI) bio-reduction was performed by mixed cultures from activated sludge under both aerobic and anaerobic conditions. The component and quantity of extracellular polymeric substances (EPS) were analyzed, and the bacterial communities were determined by high throughput sequencing. No chromium adsorption was observed by the mixed cultures in the present study. In the 12th acclimation cycle, 67% and 78% of Cr(VI) was reduced by the aerobic and anaerobic cultures in 72h, respectively. More EPS were extracted from control groups than that from Cr(VI)-treated groups. The diversities of bacterial communities in Cr(VI)-treated groups were lower than those in control groups. Proteobacteria, Firmicutes, Chloroflexi and Actinobacteria were the most dominant phyla in the inoculum sample. But, all samples were dominated by Proteobacteria after a culture of 36days with Cr(VI). While the relative abundance of Citrobacter in cultures was significantly decreased by Cr(VI), the relative abundance of Enterobacter was remarkably increased by Cr(VI).
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Received: 20 May 2016
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