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| Shifts of microbial communities during Fe(II) oxidation coupled to nitrate reduction in paddy soil |
| CHEN Peng-cheng1,2,3, LI Xiao-min2, LI Fang-bai2 |
1. Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China;
2. Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, China;
3. Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract A paddy soil which was collected from South China was cultivated in different treatments (Soil, Soil+Fe(Ⅱ), Soil+NO3- and Soil+Fe(Ⅱ)+NO3-) at circumneutral pH under anoxic conditions. The objectives were to investigate the transformations of Fe(Ⅱ) and nitrate as well as the shifts of composition and diversity of microbial communities during these processes. The results revealed that Fe(Ⅱ) could not be oxidized in the treatment of Soil+Fe(Ⅱ), but only be oxidized in the treatment of Soil+Fe(Ⅱ)+NO3-. Meanwhile, the presence of Fe(Ⅱ) slowed down the NO3- reduction. Illumina high throughput sequencing was used to profile the diversity and abundance of microbial communities over time. The results showed that no significant difference of microbial communities between treatments of Soil+Fe(Ⅱ) and Soil. Pseudogulbenkiania, Flavobacterium and Rhodocyclus gradually became the dominant genera in the treatment of Soil+NO3-. Zoogloea, Geothrix, Sunxiuqinia and Vulcanibacillus were the most abundant genera in the treatment of Soil+Fe(Ⅱ)+NO3-, which was a mixture of nitrate reducers, Fe(Ⅱ) oxidizers and Fe(Ⅲ) reducers.
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Received: 08 May 2016
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