|
|
Lipid accumulation of Chlorella pyrenoidosa under mixotrophic culture using sodium acetate |
LIU Li-jun, ZHAO Yuan, ZHANG Heng-feng, WANG Xiao-yu, LIANG Wen-yan |
Beijing Key Lab for Source Control Technology of Water Pollution, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China |
|
|
Abstract The neutral lipid (NL) accumulation of Chlorella pyrenoidosa during the mixotrophic growth was studied by using sodium acetate as the organic carbon source and Nile-Red three dimensional fluorescence-regional volume integration to determine the NL content. The differences of biomass productivity, total lipid productivity, and fatty acids of lipids between mixotrophic and autotrophic culture were also investigated. The results showed that C. pyrenoidosa entered the logarithmic growth phase immediately and grew fast after sodium acetate adding, with the NL accumulation simultaneously being triggered. The concentration of 5g/L sodium acetate could not only promote the microalgal growth and the continuous NL accumulation, but also avoid the environmental stress caused by the excessive organic carbon to algae. After 12-day cultivation, the NL content per cell, biomass production, and total lipid productivity were 4.5, 2.1, and 3.1times those of the autotrophic control samples, respectively. Under the dosage of 5g/L sodium acetate, the fatty acids of lipid mainly consisted of saturated fatty acid C16:0 (22.42%) and unsaturated fatty acids C18:1 (31.58%) and C18:2 (10.89%).
|
Received: 19 June 2016
|
|
|
|
|
[1] |
Wang J H, Yang H Z, Wang F. Mixotrophic cultivation of microalgae for biodiesel production: status and prospects [J]. Applied Biochemistry and Biotechnology, 2014,172(7):3307-3329.
|
[2] |
Slade R, Bauen A. Micro-algae cultivation for biofuels: Cost, energy balance, environmental impacts and future prospects [J]. Biomass & Bioenergy, 2013,53(2):29-38.
|
[3] |
Park K C, Whitney C, McNichol J C, et al. Mixotrophic and photoautotrophic cultivation of 14 microalgae isolates from Saskatchewan, Canada: potential applications for wastewater remediation for biofuel production [J]. Journal of Applied Phycology, 2011,24(3):339-348.
|
[4] |
Wang H Y, Xiong H R, Hui Z L, et al. Mixotrophic cultivation of Chlorella pyrenoidosa with diluted primary piggery wastewater to produce lipids [J]. Bioresource Technology, 2012,104(1):215-220.
|
[5] |
Siaut M, Cuiné S, Cagnon C, et al. Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves [J]. BMC Biotechnology, 2011,11(10):1644-1649.
|
[6] |
Liang Y N, Sarkany N, Cui Y. Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions [J]. Biotechnology Letters, 2009, 31(7):1043-1049.
|
[7] |
黄惠莹.污水厂剩余污泥水解酸化液发酵产氢试验研究 [D]. 广州:华南理工大学, 2011.
|
[8] |
梁文艳,王 珂,阮铃铃,等.电氧化杀藻过程中藻细胞活性变化的研究 [J]. 环境科学, 2010,31(6):1464-1469.
|
[9] |
Rai M P, Nigam S, Sharma R. Response of growth and fatty acid compositions of Chlorella pyrenoidosa under mixotrophic cultivation with acetate and glycerol for bioenergy application [J]. Biomass and Bioenergy, 2013,58(21):251-257.
|
[10] |
Isleten-Hosoglu M, Gultepe I, Elibol M. Optimization of carbon and nitrogen sources for biomass and lipid production by Chlorella saccharophila under heterotrophic conditions and development of Nile red fluorescence based method for quantification of its neutral lipid content [J]. Biochemical Engineering Journal, 2012,61(4):11-19.
|
[11] |
王利兵,于海燕,贺晓辉,等.生物柴油树种油脂脂肪酸组成对燃料特性的影响 [J]. 燃料化学学报, 2012,40(4):397-404.
|
[12] |
赫冬梅,王 娜,孙凯峰,等.葡萄糖和乙酸钠对湛江等鞭金藻兼养生长的影响 [J]. 生态科学, 2012,31(2):220-223.
|
[13] |
卢复中.有机化合物的BOD COD值 [J]. 建筑技术通讯:给水排水, 1980,(6):46-47.
|
[14] |
王 娜,葛 飞,吴秀珍,等.藻类及其胞外分泌物对净水工艺的影响 [J]. 水处理技术, 2010,36(2):19-24.
|
[15] |
华汝成.单细胞藻类的培养与利用 [M]. 北京:农业出版社, 1983:135-137.
|
[16] |
Bligh E G, Dyer W J. A rapid method of total lipid extraction and purification [J]. Canadian Journal of Biochemistry and Physiology, 1959,37(8):911-917.
|
[17] |
Fowler S D, Greenspan P. Application of Nile red, a fluorescent hydrophobic probe, for the detection of neutral lipid deposits in tissue sections: comparison with oil red O [J]. Journal of Histochemistry & Cytochemistry, 1985,33(8):833-836.
|
[18] |
Elsey D, Jameson D, Raleigh B, et al. Fluorescent measurement of microalgal neutral lipids [J]. Journal of Microbiological Methods, 2007,68(3):639-642.
|
[19] |
Ren H Y, Liu B F, Kong F Y, et al. Improved Nile red staining of Scenedesmus sp. by combining ultrasonic treatment and three-dimensional excitation emission matrix fluorescence spectroscopy [J]. Algal Research, 2015,7:11-15.
|
[20] |
王 昊,高乃云,欧桦瑟,等.三维荧光分析O3和UV-C处理铜绿微囊藻过程 [J]. 中南大学学报:自然科学版, 2012,43(2):763-768.
|
[21] |
吕洪刚,张锡辉,龚纯英,等.藻类的三维荧光光谱辨别及算法研究 [J]. 中国环境科学, 2005,25(5):581-584.
|
[22] |
Qiao H J, Wang G C. Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01 [J]. Chinese Journal of Oceanology and Limnology, 2009,27(4):762-768.
|
[23] |
Shifrin N S, Chisholm S W. Phytoplankton lipids: interspecific differences and effects of nitrate, silicate and light-dark cycles [J]. Journal of Phycology, 1981,17(4):374-384.
|
[24] |
Dean A P, Sigee D C, Estrada B, et al. Using FTIR spectroscopy for rapid determination of lipid accumulation in response to nitrogen limitation in freshwater microalgae [J]. Bioresource Technology, 2010,101(12):4499-4507.
|
[25] |
Ratledge C, Wynn J P. The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms [J]. Advances in Applied Microbiology, 2002,51:1-52.
|
[26] |
车绕琼,黄 力,王 琳,等.葡萄糖对单针藻异养、兼养生长及油脂合成的影响 [J]. 中国生物工程杂志, 2015,35(11):46-51.
|
[27] |
王立柱,温皓程,邹 渝,等.产油微藻的分离、筛选及自养培养氮源、碳源的优化 [J]. 微生物学通报, 2010,37(3):336-341.
|
[28] |
Ngangkham M, Ratha S K, Prasanna R, et al. Biochemical modulation of growth, lipid quality and productivity in mixotrophic cultures of Chlorella sorokiniana [J]. Springerplus, 2011,1(1):1-13.
|
[29] |
刘晓娟,段舜山,李爱芬.不同营养因子对微藻3种培养方式生产EPA的影响 [J]. 食品研究与开发, 2006,27(8):185-188.
|
[30] |
Moon M, Kim C W, Park W K, et al. Mixotrophic growth with acetate or volatile fatty acids maximizes growth and lipid production in Chlamydomonas reinhardtii [J]. Algal Research, 2013,2(4):352-357.
|
[31] |
黄 力,贺赐安,赵 鹏,等.碳源、氮源对异养单针藻Monoraphidium sp.FXY-10油脂积累和脂肪酸组成的影响 [J]. 中国生物工程杂志, 2013,33(2):59-64.
|
[32] |
Ramalho E F S M, Filho J R C, Albuquerque A R, et al. Low temperature behavior of poultry fat biodiesel:diesel blends [J]. Fuel, 2012,93(1):601-605.
|
|
|
|