常规和倒置A<sup>2</sup>/O工艺活性污泥微生物群落结构的比较

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Abstract

To explore the reason why removal efficiency of nitrogen and phosphorus in reversed anoxic-anaerobic-oxic (A²/O) process is higher than that in conventional A²/O process, bacterial community compositions of activated sludges from the two procedures were investigated by PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) technique and cultivation method. Betaproteobacteria and Gammaproteobacteria were detected as dominant bacteria in activated sludge from both processes. In the activated sludge of the reversed A²/O process, a large amount of Nitrosospira sp., Rhodocyclus sp., and four uncultured bacteroidetes bacterium were detected by PCR-DGGE technique, while less of these bacteria were found in the conventional A²/O process. Shewanella algae sp., Klebsiella sp., and Rhodobacter sphaeroides of Gammaproteobacteria were separated from the activated sludge of reversed A²/O process by cultivation method, while they were absent from the conventional A²/O process. The diversity of these above-mentioned bacteria species could account for the higher removal efficiency of nitrogen and phosphorus in the reversed A²/O process. Additionally, scanning electron microscopy showed that the activated sludge of reversed A²/O process was relatively loose, with less Filamentous bacteria and no Nostoc.

Key words： activated sludge microbial community structure PCR-DGGE reversed A²/O process

Received: 22 July 2016

PACS:

X172

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	Articles by authors
	LI Peng
	BI Xue-jun
	WANG Jun
	RU Shao-guo

Cite this article:

LI Peng,BI Xue-jun,WANG Jun等. Microbial diversity in activated sludges of conventional and reversed A²/O processes[J]. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(3): 1137-1145.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2017/V37/I3/1137

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