The inhibition effect of three different kinds of antibiotics on the nitrogen removal performance of anammox granules was investigated by serum bottle batch tests. The antibiotics used in the experiment were penicillin G sodium, oxytetracycline HCl and polymyxin E sulphate, which performed different inhibition mechanisms. As a result, when the batch test was conducted without the addition of antibiotic, the NH₄⁺-N and NO₂^--N removal rates of the anammox granules were 0.252 and 0.375kg N/(kg VSS·d), respectively. Besides, the anammox activity of the granules was not inhibited with the penicillin G sodium concentration of 3000mg/L. However, the anammox activity of the granules was largely inhibited by oxytetracycline HCl and polymyxin E sulphate. With the oxytetracycline HCl concentrations of 50, 100, 150, 200 and 400mg/L, the NH₄⁺-N removal rates were 0.250, 0.237, 0.200, 0.117 and 0.062kg N/(kg VSS·d), respectively; The NO₂^--N removal rates were 0.324, 0.304, 0.296, 0.244 and 0.069kg N/(kg VSS·d), respectively. Meanwhile, with the polymyxin E sulphate concentrations of 30, 70, 90, 100 and 300mg/L, the NH₄⁺-N removal rates were 0.230, 0.134, 0.094, 0.022 and 0.007kg N/(kg VSS·d), respectively; The NO₂^--N removal rates were 0.351, 0.203, 0.133, 0.039 and 0.004kg N/(kg VSS·d), respectively. The inhibition effects of the three kinds of antibiotics were in the order as follows: polymyxin E sulphate> oxytetracycline HCl> penicillin G sodium.