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| Effect of steric hindrance on the biodegradation of dissolved methyl-phenanthrene |
| DU Lan1, ZHANG Zhen-xuan1, ZHU Ya-xian2, ZHANG Yong1 |
1. State Key Laboratory of Marine Environmental Science of China, College of Environment and Ecology, Xiamen University, Xiamen 361102, China;
2. Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China |
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Abstract The biodegradation of dissolved phenanthrene (Phe) and methyl-phenanthrene (MP), including 1-MP, 3-MP, 4-MP and 9-MP by the bacterium Novosphingobium pentaromativorans US6-1was individually studied using synchronous fluorescence spectrometry. The biodegradation of MP was adequately described by zero-order kinetics at two concentrations, i.e., 8×10-7mol/L and 1.2×10-6mol/L. The rates of biodegradation were generally in the following order: 4-MP< 9-MP< 1-MP< 3-MP. There was a good linear relationship between the measured maximum biodegradation rates and the theoretical maximum transmembrane fluxes. The addition of NaN3 exerted little effects on the biodegradation of Phe but inhibited that of MP, with the degree of inhibition dependent on the position of the methyl substituent.
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Received: 23 August 2016
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