The soil microbial structure and community of five different kinds of contaminated soil were determined using PCR-DGGE test method, the soil microbial community and microbial similarity was measured and compared using Des matrix analy sis and B-C matrix analysis and the relationship between Zn toxicity to soil microbial structure and soil properties was also analysed by using Vegan Sorting Axis analysis. Zn pollution could decrease the number and type of microbial in soil, while it was not a simply negative correlation. The maximum value of soil microorganisms diversity indices were observed at low Zn addition level (200mg/kg) in soils, which implied that application of low concentration Zn in soils would contribute to the increased number of soil microbial community and enrich the community structure diversity, however, with the increment of Zn in soils, the microbial community structure were restrained at high Zn concentrations, the Shannon index decreased 5.14% to 17.2% among the treatments, the minimum value (5.14%) was observed in black soil from Gongzhuling and the greatest reduction was found in the paddy soil from Hangzhou. The soil microbial similarity as determined by Des matrix and B-C matrix analysis decreased significantly with Zn addition in high concentration (> 800mg/kg) in soils, with the maximum reduction of 23.3%, soil microbial community structure could be damaged after long time Zn stress in soils. Correlation analysis between the influencing factors of Zn stress and microbial community structure change in soils showed that the microbial community structure would be influenced by pH, followed by the OC, CEC in soils.