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| Effects of different sizes of CuO and potassium ethyl potassium compound pollution on soil urease and microbial diversity |
| XING Yi1,2, WANG Zhi-qiang1, LI Yi-fei1, HONG Chen1,3, SHEN Qian1, YANG Qiang1 |
1. School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China;
2. Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, Beijing 100083, China;
3. Research Center for Eco-Environmental Sciences, Chinese Academy Science, Beijing 100085, China |
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Abstract Copper oxide nanoparticles are different from the conventional copper oxide nanoparticles. Due to their small size, large specific surface area, copper oxide nanoparticles have potential hazards to the ecological environment. This study was done by adding different proportions of copper oxide nanoparticles (N CuO) and copper oxide microparticles (M CuO) to the soil (0、50、200、800、1600mgCuO /kg dry soil) to observe their effects on soil urease activity and bacterial communities. Meanwhile, the effects of N CuO and M CuO with the compound pollution of potassium ethyl xanthate (PEX) on soil microbial biomass were studied, respectively. Compared with M CuO, N CuO have stronger inhibition on the activity of urease.Only when the concentration of M CuO was 1600mg/kg, the effect of M CuO on urease activity was obvious. The inhibition of urease activity by N CuO may be related to the destruction of the cell membrane or the stimulation of the bacteria to produce reactive oxygen species, which caused the decline of bacterial cells. N CuO also has a greater influence on the bacterial community structure. Combined pollution of N CuO and PEX increased the toxicity of N CuO to soil microorganism, the activity of urease decreased greatly and the community structure of bacteria also changed greater. The mechanism of N CuO and its combined pollution on soil microbes need to be further studied. By comparing the ecological toxicity of N CuO and M CuO, the ecological toxicity of nanoparticles must be given high attention.
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Received: 02 September 2016
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