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| EOM characteristics and release of extracellular amino acids in three typical freshwater algaes |
| YANG Jing-ting1,2,3, YANG Su-wen2,3, JIN Wei-dong2,3, LIU Lei1, YAN Yu-hong2,3, MAO Qi-di1,2,3 |
1. School of Resources Environment and Chemical Engineering, Nanchang University, Nanchang 330031, China;
2. State Key Laboratory of Environmental Criteria and Risk Assessment, Beijing 100012, China;
3. State Engineering Laboratory for Lake Pollution Control and Ecological Rehabilitating Technique, Beijing 100012, China |
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Abstract The release of extracellular amino acids from algae was studied through comparative analysis of the type and composition of EOM in algae. Typical freshwater algae species, including Microcystis aeruginosa, Scenedesmus quadricauda and Navicula rhynchocephala were selected to explore the difference of the characteristic of EOM, and the quantity and composition of extracellular free amino acid and bound amino acids in algaes of stable phase, by excitation-emission matrix spectroscopy combined with parallel factor analysis (EEMs-PARAFAC) and pre-column high performance liquid chromatography (HPLC). Results showed that inorganic nitrogen can be transformed to organic nitrogen in algaes and sequentially to be released into water. The fluorescence intensity of total tryptophan and of total tyrosine in EOM was Microcystis aeruginosa > Scenedesmus quadricauda > Navicula rhynchocephala, and Navicula rhynchocephala > Scenedesmus quadricauda > Microcystis aeruginosa, respectively. The concentration of histidine, phenylalanine, glycine, and serine were higher than that of threonine, arginine and tyrosine. All of the ratios of c(DFAAs)/c(DCAAs) for the three algaes were low. Most of the amino acids existed as proteins and peptides. The ρ(DON), ρ(TDAAs) and amino acids of the Microcystis aeruginosa were the major contributor to the DON in the water body, and they are 1.31mg/L, 5.35mg/L, 69.08%, respectively. Therefore cyanobacteria bloom may release lots of amino acids into water and may increase organic nitrogen load in freshwater ecosystem.
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Received: 19 September 2016
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