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| Research on the development and properties of aerobic sludge-microalgae granular system |
| LIU Lin1,2, YE Jia-qi1, LIU Yu-hong1,2, HUANG Xu1, LIU Chao-xiang1 |
1. Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract The effect of settling time on the development and properties of algae-bacteria granular consortia,which were cultivated with aerobic granules as biological carrier,and Chlorella and Scenedesmus as seed microalgae,were investigated in this research.The results indicated that the algae-bacteria granular consortia could be successfully developed based on the aerobic granules and targeted microalgae.Although settling time could affect the start-up duration of granular system,it was not the crucial factor for the culture of granular consortia.In terms of properties of the mature granular consortia,the settling time could significantly affect the settleability,nitrogen removal ability,granular size and biodiesel yield of the consortia granules (P<0.05),while the obvious response of zeta potential,phosphate removal rate and the dominate compositions of fatty acid methyl esters in the biodiesel to the settling time was not observed in this experiment (P>0.05).The relative abundance of cyanobacteria and eukaryotic algae in all granules were about 5% and 95%,respectively.The targeted microalgae maintained dominance in the eukaryotic algal community (relative abundance>98%),and the comparative advantage of biomass between Chlorella and Scenedesmus in the granules was showed under the different condition of settling time in systems.
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Received: 06 December 2016
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| [1] |
Kwon E E, Kim S, Jeon Y J, et al. Biodiesel production from sewage sludge:new paradigm for mining energy from municipal hazardous material[J]. Environmental Science & Technology, 2012,46(18):10222-10228.
|
| [2] |
熊伟,黄云,付乾,等.微藻生物膜营养环境对微藻生长和油脂积累影响[J]. 中国环境科学, 2016,36(8):2463-2469.
|
| [3] |
Wastewater purification:aerobic granulation in sequencing batch reactors[M]. CRC Press, 2007:85-111.
|
| [4] |
隗启源,杨昌柱,濮文虹.藻类生长对好氧颗粒污泥的影响[J]. 中国给水排水, 2013,29(9):29-32.
|
| [5] |
Huang W, Li B, Zhang C, et al. Effect of algae growth on aerobic granulation and nutrients removal from synthetic wastewater by using sequencing batch reactors[J]. Bioresource Technology, 2015,179:187-192.
|
| [6] |
国家环境保护总局.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社, 2002:46-213.
|
| [7] |
Ritchie R J. Universal chlorophyll equations for estimating chlorophylls a, b, c, and d and total chlorophylls in natural assemblages of photosynthetic organisms using acetone, methanol, or ethanol solvents[J]. Photosynthetica, 2008,46(1):115-126.
|
| [8] |
Revellame E, Hernandez R, French W, et al. Biodiesel from activated sludge through in situ transesterification[J]. Journal of Chemical Technology and Biotechnology, 2010,85(5):614-620.
|
| [9] |
Wang M, Yang H, Ergas S J, et al. A novel shortcut nitrogen removal process using an algal-bacterial consortium in a photo-sequencing batch reactor (PSBR)[J]. Water Research, 2015,87:38-48.
|
| [10] |
Rüdiger W. Biosynthesis of chlorophyll b and the chlorophyll cycle[J]. Photosynthesis Research, 2002,74(2):187-193.
|
| [11] |
Qin L, Liu Y, Tay J H. Effect of settling time on aerobic granulation in sequencing batch reactor[J]. Biochemical Engineering Journal, 2004,21(1):47-52.
|
| [12] |
王然登,程战利,彭永臻,等.强化生物除磷系统中胞外聚合物的特性[J]. 中国环境科学, 2014,34(11):2838-2843.
|
| [13] |
周文广,阮榕生.微藻生物固碳技术进展和发展趋势[J]. 中国科学:化学, 2014,44(1):63-78.
|
| [14] |
刘波,孙艳,刘永红,等.产油微生物油脂生物合成与代谢调控研究进展[J]. 微生物学报, 2005,45(1):153-156.
|
| [15] |
Mondala A, Liang K, Toghiani H, et al. Biodiesel production by in situ transesterification of municipal primary and secondary sludges[J]. Bioresource Technology, 2009,100(3):1203-1210.
|
| [16] |
Zhu L, Wang Z, Shu Q, et al. Nutrient removal and biodiesel production by integration of freshwater algae cultivation with piggery wastewater treatment[J]. Water Research, 2013,47(13):4294-4302.
|
| [17] |
Georgianna D R, Mayfield S P. Exploiting diversity and synthetic biology for the production of algal biofuels[J]. Nature, 2012, 488(7411):329-335.
|
| [18] |
Gao D, Liu L, Liang H, et al. Aerobic granular sludge:characterization, mechanism of granulation and application to wastewater treatment[J]. Critical Reviews in Biotechnology, 2011,31(2):137-152.
|
| [19] |
Ramanan R, Kim B H, Cho D H, et al. Algae-bacteria interactions:evolution, ecology and emerging applications[J]. Biotechnology Advances, 2016,34(1):14-29.
|
| [20] |
Kaplan D, Wilhelm R, Abeliovich A. Interdependent environmental factors controlling nitrification in waters[J]. Water Science and Technology, 2000,42(1/2):167-172.
|
| [21] |
Liu H, Zhou Y, Xiao W, et al. Shifting nutrient-mediated interactions between algae and bacteria in a microcosm:evidence from alkaline phosphatase assay[J]. Microbiological Research, 2012,167(5):292-298.
|
| [22] |
Su Y, Mennerich A, Urban B. Municipal wastewater treatment and biomass accumulation with a wastewater-born and settleable algal-bacterial culture[J]. Water Research, 2011,45(11):3351-3358.
|
| [23] |
Hyenstrand P, Burkert U, Pettersson A, et al. Competition between the green alga Scenedesmus and the cyanobacterium Synechococcus under different modes of inorganic nitrogen supply[J]. Hydrobiologia, 2000,435(1-3):91-98.
|
| [24] |
侯爱月,李军,王昌稳,等.不同好氧颗粒污泥中微生物群落结构特点[J]. 中国环境科学, 2016,36(4):1136-1144.
|
| [25] |
Liu L, You Q, Gibson V, et al. Treatment of swine wastewater in aerobic granular reactors:comparison of different seed granules as factors[J]. Frontiers of Environmental Science & Engineering, 2015,9(6):1139-1148.
|
| [26] |
杨州,孔繁翔,史小丽,等.萼花臂尾轮虫培养滤液对铜绿微囊藻,斜生栅藻和小球藻群体形成及生长的影响[J]. 应用生态学报, 2005,16(6):1138-1141.
|
|
|
|
