紫外线消毒对大肠杆菌的损伤及复苏的研究

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Abstract

In this study,the photoreactivation and dark repair of Escherichia coli (E.coli) was investigated.The effects of ultraviolet (UV) disinfection on membrane integrity,adenosine triphosphate (ATP) and nucleic acid (DNA,RNA) were further analyzed.The aim of our study was to explore the response pattern of E.coli to UV disinfection by combination of SOS response mechanism of recA gene.The results showed that the inactivation efficiency of E.coli reached 5.63-log at a UV dose of 20mJ/cm².When the light exposure time and dark repair time was prolonged to 24h after a UV dose of 20mJ/cm²,the percentages of photoreactivation and dark repair observed for E.coli were 0.018% and 0.00042%,respectively.The ability of photoreactivation of E.coli was higher than that of dark repair.The DNA damage depended on the fragment length of genes during UV disinfection.The longer the 16s rRNA gene was,the more seriously damage occurred.Conventional UV doses in wastewater treatment did not affect the amount of total ATP and membrane integrity,which provided basic guarantee for the reactivation of E.coli after UV disinfection.UV disinfection caused serious damage in recA RNA.When UV dose reached 50mJ/cm²,E.coli lost the SOS response mechanism.Hence,little reactivation was observed using the culture method at a UV dose of 80mJ/cm².The lost of recA RNA could be used as an indicator for the occurrence of the irreversible injury to microorganism.

Key words： UV reactivation membrane integrity ATP DNA RNA

Received: 15 November 2016

PACS:	X52
	X505

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	Articles by authors
	XU Li-mei
	XU Peng-cheng
	ZHANG Chong-miao
	WANG Xiao-chang

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XU Li-mei,XU Peng-cheng,ZHANG Chong-miao等. Studies on the injury and reactivation of Escherichia coli under ultraviolet disinfection[J]. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(7): 2639-2645.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2017/V37/I7/2639

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