甲醛复合PM<sub>2.5</sub>致小鼠血液毒性的研究

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Abstract

In order to explore the influence and the underlying mechanism of FA and PM_2.5on hepatotoxicity,we conducted systematic research from three aspects,including toxicity of blood,hematopoietic organs (bone marrow,spleen) and myeloid progenitor.Male Blab/c mice were taken as experimental object,and were randomly divided into four groups:the control group;PM_2.5-exposure group;FA-exposure group;PM_2.5+FA-exposure group.Blood samples were firstly collected from mice caudal vein to conduct the complete blood count.Then the histopathological change of bone marrow and spleen (H&E staining) were observed.Furthermore,the oxidative damage (ROS,GSH),DNA damage (DPC,8-OH-dG) and apoptosis (caspase-3) in bone marrow,spleen and myeloid progenitor were detected.Results showed different degree of pathological changes in the bone marrow,spleen;compared with control group,oxidative damage,DNA damage,cell apoptosis level all demonstrated a rising trend in PM_2.5,FA,PM_2.5+FA group.In conclusion,an exposure combined formaldehyde and PM_2.5 could cause hepatotoxicity in mice,with a possible mechanism from oxidative stress as well as its downstream DNA damage.

Key words： PM_2.5 formaldehyde combined exposure hepatotoxicity

Received: 02 December 2016

PACS:

X174

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	Articles by authors
	GE Jing
	GUO Qing
	JIANG Qing-ying
	LU Mei-ling
	ZHAO Yun
	ZHU Yu-qing
	YANG Xu
	LI Rui

Cite this article:

GE Jing,GUO Qing,JIANG Qing-ying等. Formaldehyde and PM_2.5 induced hepatotoxicity in mice[J]. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(7): 2740-2748.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2017/V37/I7/2740

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