稀土开采对土壤细菌群落组成与多样性的影响

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Abstract

Experiment was designed examine impacts of rare earth mining on soil ecosystem health in Changting. Soil samples were taken from depths of 0~20cm in pre-and post-mined areas (rented earth pits, heap-leaching mine area). Microbial DNA was extracted from soil samples and then 16S rDNA gene sequencing was carried out using Illumina MiSeq equipment. Examination of bacteria species present showed the microbial population was dominated by the level of phylum, class, order, families and genus and these were Firmicutes, Bacilli, Lactobacillales, Enterococcaceae, Enterococcus. The phylogenetic tree showed there were certain genetic relationships among Firmicutes, Proteobacteria, Actinobactera, Bacteroidetes, Verrucomicrobia, Acidobacteria, Nitrospirae, Cyanobacteria, Thermi. Alpha diversity index was calculated based on operational taxonomic units (OTUs). Results indicated that soil bacterial diversity and taxonomic composition distribution changed significantly in post-mining areas compared to control areas. However, the predominant bacteria in the soil communities did not change.

Key words： rare earth soil bacteria 16S rDNA community diversity phylogenetic tree

Received: 21 March 2017

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X17

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	Articles by authors
	WANG You-sheng
	HOU Xiao-long
	CAI Li-ping
	MA Xiang-qing

Cite this article:

WANG You-sheng,HOU Xiao-long,CAI Li-ping等. Impacts of rare earth mining on soil bacterial community composition and biodiversity[J]. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(8): 3089-3095.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2017/V37/I8/3089

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