|
|
advanced Fenton oxidation treatment of tetracycline resistance genes in effluent discharged from biological wastewater treatment |
ZENG Ping1, LIU Shi-yue2, ZHANG Jun-ke3, SONG Yong-hui1, LIU Rui-xia1, LIU Yang4 |
1. Department of Urban Water Environmental Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;
2. School of Water Resources and Environment, China University of Geosciences, Beijing 100083, China;
3. School of Chemical & Environmental Engineering, China University of Mining and Technology(Beijing), Beijing 100083, China;
4. Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta, 7-263, Canada, China |
|
|
Abstract Fenton oxidation was used to remove tetracycline resistance genes (tet genes:tetL and tetE) from wastewater. Changes in the relative abundance of tet genes and the bacterial community structure were monitored using quantity polymerase chain reaction (q-PCR) and denaturing gradient gel electrophoresis (DGGE). The highest removal efficiencies for tetracycline resistance genes and 16SrRNA were achieved at the optimal conditions, including the reaction time of 10min, pH of 5, H2O2/Fe2+ molar ratio of 8 and H2O2 concentration of 0.2mol/L. DGGE analysis showed remarkable changes in the bacterial community diversities after Fenton oxidation. However, no significant correlation was observed either between the removal of tet genes and the parameters used in Fenton oxidation, or between the removal of tet genes and the change in the bacterial community structures. These results may suggested that the removal of tet genes may not only attribute to the removal of tetracycline resistance bacteria. Future investigations may be necessary to further understand the removal mechanisms in details.
|
Received: 10 February 2017
|
|
|
|
|
[1] |
Pruden A, Pei R, Storteboom H, et al. Antibiotic resistance genes as emerging contaminants:studies in northern Colorado[J]. Environmental Science & Technology, 2006,40(23):7445-7450.
|
[2] |
Baquero, F., Martinez, J. L., Canton, R. 2008. Antibiotics and antibiotic resistance in water environments[J]. Current Opinion Biotechnol, 19(3):260-265.
|
[3] |
Zhang X X, Zhang T, Fang H H. 2009. Antibiotic resistance genes in water environment[J]. Applied microbiology and biotechnology, 82(3):397-414
|
[4] |
Pei R, Kim S C, Carlson K H, et al. 2006. Effect of river landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG)[J]. Water Research, 40(12):2427-2435.
|
[5] |
邹世春,朱春敬,贺竹梅,等.2009.北江河水中抗生素抗性基因污染初步研究[J]. 生态毒理学报, 4(05):655-660.
|
[6] |
Xu J, Xu Y, Wang H, et al. Occurrence of antibiotics and antibiotic resistance genes in a sewage treatment plant and its effluent-receiving river[J]. Chemosphere, 2015,119:1379-1385.
|
[7] |
沈群辉,冀秀玲,傅淑珺,等.黄浦江水域抗生素及抗性基因污染初步研究[J]. 生态环境学报, 2012,21(10):1717-1723.
|
[8] |
Jiang L, Hu X, Xu T, et al. 2013. Prevalence of antibiotic resistance genes and their relationship with antibiotics in the Huangpu River and the drinking water sources, Shanghai, China[J]. Science of the Total Environment, 458-460:267-272.
|
[9] |
Lin L, Yuan K, Liang X, et al. Occurrences and distribution of sulfonamide and tetracycline resistance genes in the Yangtze River Estuary and nearby coastal area[J]. Marine Pollution Bulletin, 2015,110(1):304-310.
|
[10] |
Kim S, Jensen J N, Aga D S, et al. Tetracycline as a selector for resistant bacteria in activated sludge[J]. Chemosphere, 2007, 66(9):1643-1651.
|
[11] |
Segura P A, Francois M, Gagnon C, et al. Review of the occurrence of anti-infectives in contaminated wastewaters and natural and drinking waters[J]. Environmental Health Perspectives, 2009,117(5):675-684.
|
[12] |
Xi C, Zhang Y, Marrs C F, et al. Prevalence of antibiotic resistance in drinking water treatment and distribution systems[J]. Applied Environmental Microbiology, 2009,75(17):5714-5718.
|
[13] |
Huang X, Liu C, Li K, et al. Occurrence and distribution of veterinary antibiotics and tetracycline resistance genes in farmland soils around swine feedlots in Fujian Province, China[J]. Environmental Science and Pollution Research International, 2013,20(12):9066-9074.
|
[14] |
Rizzo L, Manaia C, Merlin C, et al. 2013. Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment:a review[J]. Science of the Total Environment, 447:345-360.
|
[15] |
Tao C W, Hsu B M, Ji W T, et al. Evaluation of five antibiotic resistance genes in wastewater treatment systems of swine farms by real-time PCR[J]. Science of the Total Environment, 2014, 496:116-121.
|
[16] |
Huang M H, Zhang W, Liu C, et al. Fate of trace tetracycline with resistant bacteria and resistance genes in an improved AAO wastewater treatment plant[J]. Process Safety and Environmental Protection, 2015,93:68-74.
|
[17] |
Rodriguez-Mozaz S, Chamorro S, Marti E, et al. 2015. Occurrence of antibiotics and antibiotic resistance genes in hospital and urban wastewaters and their impact on the receiving river[J]. Water Research, 69:234-242.
|
[18] |
刘苗苗,张昱,李栋,等.制药废水受纳河流中四环素抗药基因及微生物群落结构变化研究[J]. 环境科学学报, 2010,30(8):1551-1557.
|
[19] |
吴楠,乔敏,朱永官.猪场土壤中5种四环素抗性基因的检测和定量[J]. 生态毒理学报, 2009,4(5):705-710.
|
[20] |
代敏,王红宁,吴琦.2005.PCR和核酸探针检测猪源沙门氏菌四环素耐药基因tetC的研究[J]. 畜牧兽医学报, 2005, 36(5):482-485.
|
[21] |
Kim S, Park H, Chandran K. Propensity of activated sludge to amplify or attenuate tetracycline resistance genes and tetracycline resistant bacteria:a mathematical modeling approach[J]. Chemosphere, 2010,78(9):1071-1077.
|
[22] |
Munir M, Wong K, Xagoraraki I. Release of antibiotic resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan[J]. Water Research, 2011,45(2):681-693.
|
[23] |
Xia S, Jia R, Feng F, et al. Effect of solids retention time on antibiotics removal performance and microbial communities in an A/O-MBR process[J]. Bioresource Technology, 2012,106:36-43.
|
[24] |
Zhang W, Huang M H, Qi F F, et al. Effect of trace tetracycline concentrations on the structure of a microbial community and the development of tetracycline resistance genes in sequencing batch reactors[J]. Bioresource Technology, 2013,150:9-14.
|
[25] |
Wang J, Mao D, Mu Q, et al. Fate and proliferation of typical antibiotic resistance genes in five full-scale pharmaceutical wastewater treatment plants[J]. Science of the Total Environment, 2015,526:366-373.
|
[26] |
郑加玉,刘琳,高大文,等.四环素抗性基因在人工湿地中的去除及累积[J]. 环境科学, 2013,34(8):3102-3107.
|
[27] |
闫雷,闫璐,孙睿,等.碱预处理对污泥中抗生素抗性基因丰度的影响[J]. 中国给水排水, 2014,30(19):99-102.
|
[28] |
谭国赣,杜勇立,黄满红,等.污泥深度脱水对四环素耐药菌和耐药基因的去除特性[J]. 上海师范大学学报(自然科学版), 2014,43(2):175-179.
|
[29] |
Auerbach E A, Seyfried E E, McMahon K D. Tetracycline resistance genes in activated sludge wastewater treatment plants[J]. Water Research, 2007,41(5):1143-1151.
|
[30] |
庄耀,任洪强,耿金菊,等.混凝法去除城市生活污水中抗性基因[J]. 环境工程学报, 2014,8(12):5105-5110.
|
[31] |
秦乐乐.基于芬顿原理的高级氧化技术去除垃圾渗滤液中难降解有机物的研究[D]. 北京:北京交通大学, 2015.
|
[32] |
宿程远,李伟光,黄智,等.蒽醌类物质经多相类芬顿预处理前后对活性污泥特性的影响[J]. 中国环境科学, 2015,35(8):2351-2359.
|
[33] |
Ng L K, Martin I, Alfa M, et al. Multiplex PCR for the detection of tetracycline resistant genes[J]. Molecular and cellular probes, 2001,15(4):209-215.
|
[34] |
Michael I, Hapeshi E, Michael C, et al. Solar photo-Fenton process on the abatement of antibiotics at a pilot scale:Degradation kinetics, ecotoxicity and phytotoxicity assessment and removal of antibiotic resistant enterococci[J]. Water Research, 2012,46(17):5621-5634.
|
[35] |
李春娟.芬顿法和类芬顿法对水中污染物的去除研究[D]. 哈尔滨:哈尔滨工业大学, 2009.
|
|
|
|