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| Microbial source tracking of fecal contamination in the Pearl River Delta region |
| ZHANG Yang1,2, WU Ren-ren2,3, ZHANG Yi-min1,4, WU Bing-wen5, WU Xiao-qing2,3, CHEN Zhong-ying2,3, LI Kai-ming2,3 |
1. College of Resources and Environment Engineering, Wuhan University of Technology, Wuhan 430070, China;
2. South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou 510655, China;
3. The key Laboratory of Water and Air Pollution Control of Guangdong Province, Guangzhou 510655, China;
4. College of Resources and Environment Engineering, Wuhan University of Science and Technology, Wuhan 430081, China;
5. School Environmental Science and Engineering, Zhejiang GongShang University, Hangzhou 310000, China |
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Abstract Identification of the host source of microbial contaminants accurately is the basis for targeted treatment in water. To analyze the feature of pollution in the Pearl River Delta Region, qPCR method was applied to choose the suitable specific bacteroides primer, and culture-method was combined which detected the Fecal coliform and Escherichia coli to assess the water quality and the sources of fecal pollution in target area. The results showed that human-specific marker qHS601F/qBac725R, ruminant-specific marker BacB2-590F/Bac708Rm, porcine-specific marker Bac41F/Bac163R and chicken-specific marker qC160F-HU/qBac265R-HU had higher sensitivity and specificity in the Pearl River Delta region. 14 sampling sites were polluted by human, ruminant and poultry fecal. The intensity of pollution by different source was found to be human > ruminant > poultry. And the concentration of qHS601F/qBac725R had a significant correlationship with Fecal coliform, Escherichia coli and EC23S857F/EC23S857R (P<0.05).It reflects that the major contribute of pollutant source was human.
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Received: 07 March 2017
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