Fe (Ⅱ)对反硝化过程及其功能微生物群落的影响

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Abstract Paddy soil was collected from Southern China and used as an inoculum in an anoxic experiment to investigate the effect of Fe(Ⅱ) oxidation on denitrification and associated microbial communities. The results showed that the presence of Fe(Ⅱ) slowed down the NO₃^- reduction, but facilitated the NO₂^- reduction and N₂O formation; meanwhile Fe(Ⅱ) oxidation was observed only in the Soil+Fe(Ⅱ)+NO₃^- treatment. The real-time quantitative PCR analysis demonstrated that the presence of Fe(Ⅱ) increased the copy numbers of nitrite reduction gene (nirS) and nitrous oxide (N₂O) reduction gene (nosZ), but inhibited the activity of membrane nitrate reduction gene (narG). The results of high-throughput sequencing and clone library exhibited that the presence of Fe(Ⅱ) significantly influences the microbial communities affiliated with periplasmic nitrate reduction gene (napA). The most abundant napA-based nitrate reducer was Dechloromonas in the Soil+NO₃^- treatment, while those were Azonexus, Dechloromonas and Azospira in the Soil+Fe(Ⅱ)+NO₃^- treatment. In summary, Fe(Ⅱ) significantly influences the denitrification process and the associated microbial communities in anoxic paddy soil. This can provide better understanding of the relationship between the nitrogen cycling and the iron transformation in the red paddy soil from South China.

Key words： Fe (Ⅱ) denitrification functional gene functional microbial communities paddy soil

Received: 05 June 2017

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X172

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	LI Shuang
	LI Xiao-min
	LI Fang-bai

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LI Shuang,LI Xiao-min,LI Fang-bai. Effect of Fe(Ⅱ) on denitrification and associated functional microbial communities[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(1): 263-274.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2018/V38/I1/263

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