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Effect of aeration rate on granular sludge for biodiesel production |
CHI Xia, LI An-jie, LI Mei-xi |
Key Laboratory of Water and Sediment Sciences of Ministry of Education/State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China |
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Abstract Aerobic granular sludge for sugar-containing wastewater treatment is a promising raw material for biodiesel production, which also could reduce the environmental contamination of wastewater discharge and convert excessive granular sludge into valuable resources. In this study, the effect of aeration rate on the biodiesel production from granular sludge was investigated. The results indicated that granules cultivated at different aeration rates had different morphologies and the microbial structures of bacteria and fungi. The biodiesel yield reached the highest level[(48.62 ±1.36)mg/g SS] when the aeration rate was 167L/(min·m3). The aeration rate with 167L/(min·m3) resulted in the dominance of the white filamentous fungi Dipodascus (8.57%) in granular sludge, thereby enhancing the lipid accumulation of granular sludge and changing the biodiesel constituent produced from the sludge. The growth of Dipodascus in granular sludge possibly led to a great increase in linoleic acid (C18:2). Obviously, the biomass and microbial community could be affected by aeration rate which determined the content and constituent of FAMEs produced from granular sludge. The yield and constituent of biodiesel produced from excessive granular sludge could be improved by controlling the aeration conditions of granulation system in actual applications.
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Received: 18 July 2017
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[1] |
Mondala A, Liang K, Toghiani H, et al. Biodiesel production by in situ transesterification of municipal primary and secondary sludges[J]. Bioresource Technology, 2009,100(3):1203-1210.
|
[2] |
刘琳,叶嘉琦,刘玉洪,等.好氧污泥-微藻耦合颗粒的培养及特性研究[J]. 2017,37(7):2536-2541.
|
[3] |
Pastore C, Lopez A, Lotito V, et al. Biodiesel from dewatered wastewater sludge:a two-step process for a more advantageous production[J]. Chemosphere, 2013,92(6):667-673.
|
[4] |
杨岸明.城市污水处理厂曝气节能方法与技术[D]. 北京工业大学, 2012.
|
[5] |
Ahmed S U, Singh S K, Pandey A, et al. Fatty acid profiling during microbial lipid production under varying pO2 and impeller tip speeds[J]. Applied Biochemistry and Biotechnology, 2008, 151(2):599-609.
|
[6] |
Philipp Gerhardt, Murray R G E, et al. Methods for general and molecular bacteriology[M]. American Society for Microbiology, 1994.
|
[7] |
HJ535-2009《水质氨氮的测定-纳氏试剂分光光度法》[S].
|
[8] |
APHA, 2005. Standard methods for the examination of water and wastewater, twenty first ed[J]. American Public Health Administration, Washington, 2005.
|
[9] |
Sun Q, Li A J, Li M X, et al. Effect of pH on biodiesel production and the microbial structure of glucose-fed activated sludge[J]. Int Biodeter Biodegr, 2015,104:224-230.
|
[10] |
王怡,白小娟,艾怡霏,等.城市污水处理厂污泥制取生物柴油的试验研究[J]. 中国给水排水, 2014,(1):14-17.
|
[11] |
李元森.产油脂丝状真菌的筛选及其产油脂条件的优化[D]. 济南:山东轻工业学院, 2009.
|
[12] |
Bruland N, Bathe S, Willems A, et al. Pseudorhodoferax soli gen. nov., sp. nov. and Pseudorhodoferax caeni sp. nov., two members of the class Betaproteobacteria belonging to the family Comamonadaceae[J]. International Journal of Systematic & Evolutionary Microbiology, 2009,59(11):2702-2709.
|
[13] |
Dong L, Ming H, Liu L, et al. Zhizhongheella caldifontis gen. nov., sp. nov., a novel member of the family Comamonadaceae[J]. Antonie van Leeuwenhoek, 2014,105(4):755-761.
|
[14] |
Fang W, Li Y, Xue H, et al. Corticibacter populi gen. nov., sp. nov., a new member of the Comamonadaceae, from the bark of Populus euramericana[J]. International Journal of Systematic & Evolutionary Microbiology, 2015,65(10):3333-3338.
|
[15] |
Caldwell M E, Allen T D, Lawson P A, et al. Tolumonas osonensis sp. nov., isolated from anoxic freshwater sediment, and emended description of the genus Tolumonas[J]. International Journal of Systematic & Evolutionary Microbiology, 2011,61(11):2659-2663.
|
[16] |
Kuang G, Xiao A, Chen Q, et al. Characterization of PB-CS01, A novel photobacterium strain isolated from commercial pork[J]. Journal of Animal & Veterinary Advances, 2012,11(23):4292-4296.
|
[17] |
Veld G I T, Driessen A J M, Kamp J A F O D, et al. Hydrophobic membrane thickness and lipid-protein interactions of the leucine transport system of Lactococcus lactis[J]. Biochimica Et Biophysica Acta, 1991,1065(2):203-212.
|
[18] |
Contzen M, Moore E R, Blümel S, et al. Hydrogenophaga intermedia sp. nov., a 4-aminobenzenesulfonate degrading organism[J]. Systematic & Applied Microbiology, 2000,23(4):487-493.
|
[19] |
Botha A, Kock J L. The distribution and taxonomic value of fatty acids and eicosanoids in the Lipomycetaceae and Dipodascaceae[J]. Antonie van Leeuwenhoek, 1993,63(2):111-123.
|
|
|
|