氨氮对异养硝化菌<i>Acinetobactor</i> sp.活性影响及动力学特性分析

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (602 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract The strain JQ1004 with capability of heterotrophic nitrification were able to grow and metabolism under different initial ammonium concentrations in range of 0~2000mg/L.However,the strain were completely inhibited in concentration of 2500mg/L.When strain JQ1004 was cultivated at 30℃,pH 7.5,160rpm with the initial ammonium of 100,300,500,700,1000,1500,2000mg/L,the maximum specific growth rates were 0.251,0.308,0.286,0.243,0.197,0.115,0.088h^-1,and the corresponding ammonium specific removal rates reached 1.335,1.906,1.859,1.759,1.562,1.286,0.965g/g (DCW·d),respectively.Due to the inhibition of free ammonia and high-strength concentration of ammonium,the specific growth rate and degradation rate of ammonia increased at first and decreased with the increase of initial concentration of ammonia nitrogen (free ammonia).Three kinetic models (Haldane,Yano,Aiba) were fitted well to the experimental growth kinetic data with the correlation coefficients (R²) of 0.9944,0.9983 and 0.9929.For Haldane model,the values of μ_max,K_s,and K_i were 2.604h^-1,22.57mg/L,and 1445.31mg/L,respectively.The large values of K_i,far greater than that of autotrophic nitrifiers or anaerobic ammonium-oxidizing bacteria,indicated that JQ1004 had good tolerance against high ammonium concentrations.Besides,the specific growth rate reached a maximum value of 0.583h^-1 when the concentration of free ammonia was 5.436mg/L.These results indicated possible future applications of JQ1004 in removing nitrogen and organic carbon from high-strength ammonium wastewater.

Key words： heterotrophic nitrification biokinetics high-strength ammonium ammonia inhibition

Received: 06 August 2017

X703.5

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WANG Xiu-jie
	WANG Wei-qi
	LI Jun
	WANG Si-yu
	ZHANG Jing
	WEI Jia
	ZHAO Bai-hang

Cite this article:

WANG Xiu-jie,WANG Wei-qi,LI Jun等. Inhibition of initial ammonia and free ammonia nitrogen on Acinetobactor sp. and their biokinetics[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(3): 943-949.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2018/V38/I3/943

[1]	孙锦宜.含氮废水处理技术与应用[M]. 北京:化学工业出版社, 2003.
[2]	Liu J, Luo J, Zhou J, et al. Inhibitory effect of high-strength ammonia nitrogen on bio-treatment of landfill leachate using EGSB reactor under mesophilic and atmospheric conditions[J]. Bioresource Technology, 2012,113:239-243.
[3]	Wang S, Wang L, Deng L, et al. Performance of autotrophic nitrogen removal from digested piggery wastewater[J]. Bioresource Technology, 2017,241:465-472.
[4]	Ahmad M, Liu S, Mahmood N, et al. Synergic adsorption-biodegradation by an advanced carrier for enhanced removal of high-strength nitrogen and refractory organics[J]. ACS Applied Materials & Interfaces, 2017,9(15):13188-13200.
[5]	Ren Y, Yan L, Hao G, et al. Shortcut nitrification treatment of high strength ammonia nitrogen wastewater by aerobic granular sludge[J]. Journal of the Chemical Society of Pakistan, 2016,38(6):1222-1222.
[6]	Sun X, Zhang H, Cheng Z. Use of bioreactor landfill for nitrogen removal to enhance methane production through ex situ simultaneous nitrification-denitrification and in situ denitrification[J]. Waste Management, 2017,66:97-102.
[7]	Tang C J, Zheng P, Wang C H, et al. Performance of high-loaded ANAMMOX UASB reactors containing granular sludge[J]. Water Research, 2011,45(1):135-144.
[8]	Verstraete W, Alexander M. Heterotrophic nitrification by Arthrobacter sp[J]. Journal of Bacteriology, 1972,110(3):955-961.
[9]	Robertson L A, Kuenen J G. Thiosphaera pantotropha gen. nov. sp. nov., a facultatively anaerobic, facultatively autotrophic sulphur bacterium[J]. Microbiology, 1983,129(9):2847-2855.
[10]	Padhi S K, Tripathy S, Mohanty S, et al. Aerobic and heterotrophic nitrogen removal by Enterobacter cloacae CF-S27 with efficient utilization of hydroxylamine[J]. Bioresource Technology, 2017,232:285-296.
[11]	He T, Li Z, Sun Q, et al. Heterotrophic nitrification and aerobic denitrification by Pseudomonas tolaasii Y-11without nitrite accumulation during nitrogen conversion[J]. Bioresource Technology, 2016,200:493-499.
[12]	Yang L, Ren Y X, Liang X, et al. Nitrogen removal characteristics of a heterotrophic nitrifier Acinetobacter junii YB and its potential application for the treatment of high-strength nitrogenous wastewater[J]. Bioresource Technology, 2015,193:227-233.
[13]	Taylor S M, Yiliang H, Bin Z, et al. Heterotrophic ammonium removal characteristics of an aerobic heterotrophic nitrifying-denitrifying bacterium, Providencia rettgeri YL[J]. Journal of Environmental Sciences, 2009,21(10):1336-1341.
[14]	Joo H S, Hirai M, Shoda M. Piggery wastewater treatment using Alcaligenes faecalis strain No. 4with heterotrophic nitrification and aerobic denitrification[J]. Water Research, 2006,40(16):3029-36.
[15]	Chen J, Han Y, Wang Y, et al. Start-up and microbial communities of a simultaneous nitrogen removal system for high salinity and high nitrogen organic wastewater via heterotrophic nitrification[J]. Bioresource Technology, 2016,216:196-202.
[16]	Phatak P S, Trivedi S, Garg A, et al. Start-up of sequencing batch reactor with Thiosphaera pantotropha for treatment of high-strength nitrogenous wastewater and sludge characterization[J]. Environmental Science and Pollution Research, 2016,23(20):20065-20080.
[17]	王秀杰,王维奇,李军,等.异养硝化菌Acinetobacter sp.的分离鉴定及其脱氮特性研究[J] 中国环境科学, 2017,37(11):4241-4250.
[18]	Yan J, Wen J, Li H, et al. The biodegradation of phenol at high initial concentration by the yeast Candida tropicalis [J]. Biochemical Engineering Journal, 2005,24(3):243-247.
[19]	Medhi K, Singhal A, Chauhan D K, et al. Investigating the nitrification and denitrification kinetics under aerobic and anaerobic conditions by Paracoccus denitrificans ISTOD1[J]. Bioresource Technology, 2017,242:334-343.
[20]	Anthonisen A C, Loehr R C, Prakasam T B, et al. Inhibition of nitrification by ammonia and nitrous acid[J]. Journal-Water Pollution Control Federation, 1976,48(5):835.
[21]	Zhang S, Sha C, Jiang W, et al. Ammonium removal at low temperature by a newly isolated heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas fluorescens wsw-1001[J]. Environmental Technology, 2015,36(19):2488-2494.
[22]	Sun Z, Lv Y, Liu Y, et al. Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a novel metal resistant bacterium Cupriavidus sp. S1[J]. Bioresource Technology, 2016,220:142-150.
[23]	Joo H S, Hirai M, Shoda M. Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis No.4[J]. Journal of Bioscience and Bioengineering, 2005,100(2):184-191.
[24]	Ren Y X, Yang L, Liang X. The characteristics of a novel heterotrophic nitrifying and aerobic denitrifying bacterium, Acinetobacter junii YB[J]. Bioresource Technology, 2014,171:1-9.
[25]	Li Y, Wang Y, Fu L, et al. Aerobic-heterotrophic nitrogen removal through nitrate reduction and ammonium assimilation by marine bacterium Vibrio sp. Y1-5[J]. Bioresource Technology, 2017,230:103-111.
[26]	Deng X, Wei C, Ren Y, et al. Isolation and identification of Achromobacter sp. DN-06and evaluation of its pyridine degradation kinetics[J]. Water, Air, & Soil Pollution, 2011, 221(1-4):365.
[27]	Ouyang Y, Norton J M, Stark J M. Ammonium availability and temperature control contributions of ammonia oxidizing bacteria and archaea to nitrification in an agricultural soil[J]. Soil Biology and Biochemistry, 2017,113:161-172.
[28]	李芸,熊向阳,李军,等.膜生物反应器处理晚期垃圾渗滤液亚硝化性能及其抑制动力学分析[J]. 中国环境科学, 2016, 36(2):419-427.
[29]	Mével G, Prieur D. Heterotrophic nitrification by a thermophilic Bacillus species as influenced by different culture conditions[J]. Canadian Journal of Microbiology, 2000,46(5):465-473.