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| Study on the trans-membrane transport process of fluoranthene by Rhodococcus sp. BAP-1 |
| LI Yi1, WANG Hong-qi2, WU Xiao-xiong2, XU Jie2 |
1. College of Environment and Resource, Guangxi Normal University, Guilin 541004, China;
2. College of Water Sciences, Beijing Normal University, Beijing 100875, China |
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Abstract Trans-membrane transport of 14C-fluoranthene by Rhodococcus sp. BAP-1 was investigated by using the isotope tracing technique. The results indicated that in the presence of ATP inhibitor such as NaN3, there were no significant change on the content of 14C-fluoranthene in the cell pellet of Rhodococcus sp. BAP-1during the uptake process. Therefore, the results demonstrated that the biodegradation process would be strongly prevented if the fluoranthene could not travel across the cell membrane. Meanwhile, the results indicated that under different substrate concentrations, the transport process of 14C-fluoranthene was driven by a active transport mechanism following the intra/extra substrate concentration gradient. Also, the transport capacity of 14C-fluoranthene had no special relationship with the inoculum level. Michaelis-Menten equation was used to characterize the trans-membrane transport kinetics, according to the experimental results, pollutants might be growing as a confluent biofilm and the uptake system might have a high specific affinity, which would significantly promote the trans-membrane transport process.
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Received: 18 September 2017
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