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| Study of enzyme-reverse micelle systembyelectron spin resonance |
| PENG Xin, XU Peng-fei, DU Hao, TANG Yu, YUAN Lu, MENG Yong |
| National & Local Joint Engineering Laboratory for New Petro-chemical Materials and Fine Utilization of Resources;Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research(Hunan Normal University), Ministry of Education;Key Laboratory of the Assembly and Application of Organic Functional Molecules of Hunan Province;College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China |
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Abstract In this study, a reverse micelle system was constructed using rhamnolipid, and the properties of rhamnolipid reverse micelles and rhamnolipid reverse micelle enzyme system were studied by electron spin resonance (ESR) technique. ESR spectroscopy was used to calculate the performance. The ultrafine splitting constant in the reverse micelle system was obtained. The study showed that the criticality of rhamnolipid in n-hexane was 0.07mmol/L. By analyzing the change of rotation correlation time in ESR spectrum, the electron spin probe was explored. At the same time, the peak of the ESR spectrum reflects the amount of free radicals in the sample. By comparing the reverse micelle system, the reverse micelle enzyme system and the reverse micelle enzyme-phenol system ESR spectra showed that the rhamnolipid reversed micelle-phenol system had the most free radicals. By studying the effect of 16-nitroxyl radical stearic acid on two systems, it was speculated that the electron spin probe free radicals were localized. In the reverse micelle water core, and inferred that the electron spin probe was located in the edge region of the water core, that was, the water-water layer was combined. This study provided a solid theoretical basis for the application of the reverse micelle enzyme system.
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Received: 06 July 2018
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