重庆市河水中粪源微生物污染特征及源解析

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Abstract In this study, 13 water samples were collected from the main stem rivers in Chongqing city. The numbers of total bacteria, Streptococcus faecalis, enterococci, Bacteroides fragillis, total coliforms and Fecal coliforms were counted using traditional culturing techniques. The bacteroides were selected as specific indicator bacteria to identify the specific fecal pollution sources by using human-specific bacteroides maker (HF183) and pig-specific bacteroides maker (Pig-2-Bac). The results showed that 15.4% of the main stem river reaches in Chongqing did not reach category Ⅲ water quality in spring, and 61.5% did not reach category Ⅲ water quality in autumn. In spring, the rivers in the main urban areas were mainly polluted by human excrement, and those in the rural areas were mainly polluted by animal manure. In autumn, the main stem river reaches and the rivers in the rural areaswere polluted by human excrement. Pearson correlation analysis of indicator microorganisms showed that there were strong correlation between any two indicator of fecal streptococcus, fecal coliform and enterococci, and there was a significant correlation between enterococci and Bacteroides fragilis. The verification tests showed that pig source-specific biomarkers, pig-2-bac and human-specific markers, HF183, showed good specificity to pig manure and human feces samples, respectively. Using these two specific biomarkers to amplify DNA in water samples, it was found that in the spring all the 13 sampling sites were not contaminated by pig feces; four samples including Tangjiatuo, Chaotianmen, Jiguanshi and Hechuan were polluted by human feces.

Key words： fecal microorganisms microbial source tracking bacteroides main rivers Chongqing

Received: 27 August 2018

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X522

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	Articles by authors
	ZHANG Lin-jie
	WANG Gan-gan
	ZHANG Li-lan
	RUAN Xue-yin
	ZHOU Shao-hong
	ZHANG Dai-jun
	ZHAO Lin
	WANG Peng-fei

Cite this article:

ZHANG Lin-jie,WANG Gan-gan,ZHANG Li-lan等. Pollution characteristics and source track of fecal microorganism in the rivers across Chongqing city[J]. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(3): 1253-1260.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2019/V39/I3/1253

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