氧化石墨烯对嗜热四膜虫的毒性效应

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Abstract Effects of graphene oxide (GO) on the growth, acetylcholinesterase (AchE) activity, oxidative stress, membrane damage and apoptosis of Tetrahymena thermophila were investigated. The results showed that the concentrations higher than 32mg/L GO significantly inhibited the growth of T. thermophila (P<0.05), and the cell survival rate was less than 50%. With the increase of GO concentration (0~64mg/L), the levels of reactive oxygen species (ROS) and superoxide dismutase (SOD) in T. thermophila cells initially also increased but then decreased while the activity of AchE decreased. GO inhibited the activity of succinate dehydrogenase (SDH) and promoted the release of lactate dehydrogenase (LDH). Highest concentration of GO (64mg/L) caused cell apoptosis. Based on the results, it can be concluded that oxidative stress contributed to cytotoxicity at low and medium concentrations of GO (0~8mg/L) while at higher concentrations (32 and 64mg/L) of GO, apoptosis of Tetrahymena may be caused by GO inhibiting its growth.

Key words： graphene oxide (GO) Tetrahymena thermophila toxic mechanism oxidative stress

Received: 19 August 2018

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X172

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	Articles by authors
	LIAO Yuan-chen
	CHANG Ye-qian
	XU Chen-ke
	CUI Yi-bin
	LI Mei

Cite this article:

LIAO Yuan-chen,CHANG Ye-qian,XU Chen-ke等. Toxicity effects of graphene oxide to Tetrahymena thermophila[J]. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(3): 1299-1305.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2019/V39/I3/1299

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