In order to further enhance the immobilization capability and biodegradability of functional bacteria in bio-enhanced activated carbon process (BEAC), pore structure regulation of coal-based activated carbon was conducted by an innovative agglomerated procedure including CO₂ oxidation and depth-activation. New type carbon XHIT was consequently prepared in the present work. Characterization results suggested that the volume (0.7041cm³/g) and ratio (63.95%) of meso-porous structure in carbon XHIT were significantly improved. Immobilization capability of functional bacteria on surface of XHIT was also improved synchronously during the pore-structure regulation. The initial immobilized biomass reached up to 9.13mmol/g(P), and its multiplication rate was 2.123mmol/(g·d)(P). After depth-activation process, surface oxygen content of XHIT was improved to 9.96%, which caused the significant reduction of dissolved oxygen affinity ((0.42±0.07)mg DO/L) during the adsorption process. And this phenomenon also enhanced the dissolved oxygen utilization efficiency in biodegradation (91.28%). Based on carbon XHIT, BEAC pilot process system was established for purification of source water from Songhua River. Results shows that the removal efficiency and cumulative uptake of organic-pollutants represented by COD_Mn reached to (70.65±15.22)% and 94655.50mg·COD_Mn/(kg·Carbon), respectively, with the KBV of 39.50m³/kg.