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| Preliminary study on extraction and purification of ceramide in sewage sludge |
| DONG Yi1, ZHU Fen-fen1, ZHANG Rong-yan1, WANG Peng2, CHEN Bai-ling2, ZHANG Dong-rui1 |
1. School of Environment & Natural Resources, Renmin University of China, Beijing 100872, China;
2. Department of Chemistry, Renmin University of China, Beijing 100872, China |
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Abstract Using the freeze-dried sludge of A2O-MBR process as raw material, we firstly obtained the crude lipid in the sludge by Soxhlet extraction. Then the crude lipid was eluted in a sequence of petroleum ether-ethyl acetate 100:1, 10:1 and 7:3 (V/V) by silica gel column chromatography. Compared the eluent with ceramide standard by thin layer chromatography, it was proved that the appropriate elution gradient was petroleum ether-ethyl acetate 7:3 (V/V). Through Q Exactive bench quadrupole orbitrap high-resolution mass spectrometry, the peak area ratio of ceramide was increased from 60.88% to 98.349% in the process of column chromatography. When the sludge of A2O process was used as raw material in the same experiment, the peak area ratio of ceramide was increased from 79.06% to 94.15%.
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Received: 29 October 2018
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