曝气灌溉条件下土壤N<sub>2</sub>O排放特征及影响因子分析

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Abstract

To clarify the characteristics of soil N₂O emission and identify the main factors under aerated irrigation (AI), the soil culture experiments were conducted at 2 irrigation levels with upper soil moisture limit as 70% and 90% of field capacity and 2 dissolved oxygen (DO) levels at 5 and 40mg/L. Soil N₂O emission fluxes were monitored using static chamber-gas chromatography method and the copy number of nitrification and denitrification gene were determined using the real-time quantitative polymerase chain reaction (qPCR) technique. In addition, the main factors on soil N₂O fluxes were analyzed, including soil water filling porosity (WFPS), DO, redox potential (Eh), mineral nitrogen content (NO₃^--N and NH₄⁺-N), as well as the abundance of soil ammonia-oxidizing bacterial (AOB) and ammonia-oxidizing archaea (AOA) and denitrifier genes (narG and nosZ). Results showed that soil N₂O flux increased from the beginning, peaked at 1d after irrigation, dropped in the following 3days, and then stabilized. An increase of aeration and irrigation amount resulted in the increase of average values and peak values of soil N₂O fluxes. Irrigation caused an increase of WFPS, while a decrease of soil DO and Eh. Aeration treatments increased soil DO concentration and Eh (P<0.05), improved soil aeration. However, aeration treatments showed no significant impact on WFPS. The WFPS, Eh and NO₃^--N content were the main physical, chemical influencing factors driving soil N₂O emission under AI. The AI significantly affected AOA copy numbers. In addition, soil N₂O fluxes were significantly correlated with AOA copy number (P<0.05). The results could provide scientific support for the influential mechanism of AI on soil N₂O and the farmland N₂O emission management.

Key words： aerated irrigation N₂O emission dissolved oxygen redox potential gene copy numbers influencing factor

Received: 19 October 2018

PACS:	X511
	S275

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	LEI Hong-jun
	LIU Huan
	ZANG Ming
	PAN Hong-wei
	CHEN De-li

Cite this article:

LEI Hong-jun,LIU Huan,ZANG Ming等. Characteristics and influencing factors of N₂O emission from incubated soil under aerated irrigation[J]. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(5): 2115-2122.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2019/V39/I5/2115

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