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| Effects of long-term fertilization on the abundance of the key denitrifiers in profile of paddy soil profiles |
| CHEN Na1,2, LIU Yi2, LI Juan1, YUAN Jing2, GE Ti-da2, WU Jin-shui2, SUN Zhi-long3, XU Hua-qin1 |
1. College of Agronomy, Hunan Agriculture University, Changsha 410128, China;
2. Key Laboratory of Subtropical Agriculture Ecology, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
3. Integrated Service for Agriculture Ningxiang County Huilongpu Town, Ningxiang 410606, China |
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Abstract The aims of this study were to explore the effect of long-term fertilization on the abundance of the key denitrifiers in paddy soil profiles (0~40cm), and the core factors driving denitrifiers. Soils with non-fertilization (CK), inorganic fertilizer (NPK) and organic fertilizer (ST) were collected in Ning xiang County, Hunan Province, and real-time fluorescent quantitative PCR technology was used to analyze the abundance of narG-, nirK-and nirS-containing communities in paddy soil profile (0~10cm, 10~20cm, 20~30cm, 30~40cm) and their relationship with soil properties. The results showed that compared with CK, SOC、TN、NO3--N、NH4+-N and Olsen-P in soil profile under NPK and ST increased by 2.2%~83.6%、3.5%~58.3%、70.8%~222.1%、0.9%~83.7% and 16.5%~94.5% respectively, and pH decreased by 0.31~0.67 units. Long-term application of inorganic fertilizer and organic fertilizer increased narG, nirK, and nirS gene abundance by 0.75~7.18 times, 1.57~3.02 times, and 0.53~3.81 times, respectively. And the effect of organic fertilizer on the abundance of denitrifiers was more significant than that of inorganic fertilizer application; The abundance of narG-, nirK-and nirS-containing communities decreased gradually with soil depth increasing, which presented an obvious vertical distribution; RDA analysis showed that soil nutrients such as SOC and TN were the core factors affecting the vertical distribution of narG-, nirK-and nirS-containing populations in paddy soil, especially in the cultivated horizon, while pH was the core driving factor regulating the distribution of denitrifying bacteria in paddy field subsoil. The results can provide theoretical basis for improving soil fertility and reducing nitrogen loss and greenhouse gas emission in paddy soils.
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Received: 14 October 2018
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