|
|
|
| Measurement based on DTT method of the PM2.5 oxidative potential in Guangzhou urban area |
| ZHANG Man-man1, LI Hui-rong1, YANG Wen-da1, SUN Jia-yin1, WU Cheng1, CHENG Peng1, TANG Ming-jin2 |
1. Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution, Institute of Mass Spectrometer and Atmospheric Environment, Jinan University, Guangzhou 510632, China;
2. State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China |
|
|
|
|
Abstract Oxidative potential can be used to characterize the impact of atmospheric particulate matter on human health. The DTT assay is a method recently developed to determine the oxidation potential of particulate matter. The extraction and reaction process of the DTT method were improved, and the method was employed to measure the oxidation potential of PM2.5 (DTTm & DTTv) at an urban site in Guangzhou. The DTTm values of PM2.5 in Guangzhou in January and April 2018 were found to be (13.47±3.86), (14.66±4.49) pmol/(min·μg), and the DTTv values were measured to be (4.67±1.06), (4.45±1.02) nmol/(min·m3), respectively. DTTv showed strongly correlations with mass concentration of PM2.5, OC, EC and BC, but no correlations were found between DTTm and carbonaceous particles, implied that carbonaceous particles is not the major contributor to oxidative potential of PM2.5 in Guangzhou.
|
|
Received: 09 October 2018
|
|
|
|
|
|
| [1] |
吴兑.近十年中国灰霾天气研究综述[J]. 环境科学学报, 2012, 32(2):257-269.WU D. Hazy weather research in China in the last decade:A review[J]. Acta Scientiae Circumstantiae, 2012,32(2):257-269.
|
| [2] |
唐孝炎,张远航,邵敏.大气环境化学[M]. 北京:高等教育出版社, 2006Tang X Y, Zhang Y H, Shao M. Atmospheric Environmental Chemistry.[M]. Beijing:Higher Education Press, 2006.
|
| [3] |
Sint T, Donohue J F, Ghio A J. Ambient air pollution particles and the acute exacerbation of chronic obstructive pulmonary disease[J]. Inhalation Toxicology, 2008,20(1):25-29.
|
| [4] |
Fajersztajn L, Veras M, Barrozo L V, et al. Air pollution:a potentially modifiable risk factor for lung cancer.[J]. Nature Reviews Cancer, 2013,13(9):674.
|
| [5] |
Pascal L, Pascal M, Stempfelet M, et al. Ecological study on hospitalizations for cancer, cardiovascular, and respiratory diseases in the industrial area of Etang-de-Berre in the South of France[J]. Journal of Environmental and Public Health, 2013.
|
| [6] |
Raaschounielsen O, Andersen Z J, Beelen R, et al. Air pollution and lung cancer incidence in 17 European cohorts:prospective analyses from the European Study of Cohorts for Air Pollution Effects (ESCAPE).[J]. Lancet Oncology, 2013,14(9):813-822.
|
| [7] |
芮魏.PM2.5污染特征及其对BEAS-2B和EA.hy926细胞的分子毒性研究[D]. 北京.中国科学院大学, 2015.Rui W. Characteristics of PM2.5 pollution and its molecular toxicity to BEAS-2B and EA.hy926 cells[D]. Beijing:University of Chinese Academy of Sciences, 2015.
|
| [8] |
Cho A K, Sioutas C, Miguel A H, et al. Redox activity of airborne particulate matter at different sites in the Los Angeles Basin[J]. Environmental Research, 2005,99(1):1-47.
|
| [9] |
Tao F, Gonzalezflecha B, Kobzik L. Reactive oxygen species in pulmonary inflammation by ambient particulates. Free Rad. Biol. Med[J]. Free Radical Biology & Medicine, 2003,35(4):327-340.
|
| [10] |
Kuenzli S, Shi T, Goetschi T, et al. Beyond the mass:oxidative properties of PM2.5 in the European community respiratory health survey (ECRHS)[J]. Epidemiology, 2004,15(15):S43.
|
| [11] |
Susanna E, Rossana T, Mara L, et al. Possible molecular mechanisms linking air pollution and asthma in children[J]. BMC Pulmonary Medicine, 2014,14(1):31-31.
|
| [12] |
Gonzálezflecha B. Oxidant mechanisms in response to ambient air particles.[J]. Molecular Aspects of Medicine, 2004,25(1):169-182.
|
| [13] |
Sauvain J J, Rossi M J, Riediker M. Comparison of three acellular tests for assessing the oxidation potential of nanomaterials[J]. Aerosol Science and Technology, 2013,47(2):218-227.
|
| [14] |
Hedayat F, Stevanovic S, Miljevic B, et al. Review-Evaluating the molecular assays for measuring the oxidative potential of particulate matter[J]. Chemical Industry and Chemical Engineering Quarterly, 2014,21(1/2):201-210.
|
| [15] |
Xiong Q, Yu H, Wang R, et al. Rethinking dithiothreitol-based particulate matter oxidative potential:measuring dithiothreitol consumption versus reactive oxygen species generation[J]. Environmental science & technology, 2017,51(11):6507-6514.
|
| [16] |
Kumagai Y, Koide S, Taguchi K, et al. Oxidation of proximal protein sulfhydryls by phenanthraquinone, a component of diesel exhaust particles[J]. Chemical Research in Toxicology, 2002,15(4):483-489.
|
| [17] |
Verma V, Rico-Martinez R, Kotra N, et al. Contribution of water-soluble and insoluble components and their hydrophobic/hydrophilic subfractions to the reactive oxygen species-generating potential of fine ambient aerosols[J]. Environmental Science & Technology, 2012,46(20):11384-11392.
|
| [18] |
Fang T, Verma V, Bates J T, et al. Oxidative potential of ambient water-soluble PM2.5 in the southeastern United States:contrasts in sources and health associations between ascorbic acid (AA) and dithiothreitol (DTT) assays[J]. Atmospheric Chemistry and Physics, 2016,16(6):3865-3879.
|
| [19] |
Verma V, Fang T, Xu L, et al. Organic aerosols associated with the generation of reactive oxygen species (ROS) by water-soluble PM2.5[J]. Environmental science & technology, 2015,49(7):4646-4656.
|
| [20] |
Charrier J G, Richards-Henderson N K, Bein K J, et al Oxidant production from source-oriented particulate matter -Part 1:Oxidative potential using the dithiothreitol (DTT) assay[J]. Atmospheric Chemistry and Physics, 2015,15(5):2327-2340.
|
| [21] |
Fang T, Verma V, Guo H, et al. A semi-automated system for quantifying the oxidative potential of ambient particles in aqueous extracts using the dithiothreitol (DTT) assay:results from the Southeastern Center for Air Pollution and Epidemiology (SCAPE)[J]. Atmospheric Measurement Techniques Discussions, 2014,7(7):7245-7279.
|
| [22] |
Dou J, Lin P, Kuang B Y, et al. Reactive oxygen species production mediated by humic-like substances in atmospheric aerosols:enhancement effects by pyridine, imidazole, and their derivatives[J]. Environmental Science & Technology, 2015,49(11):6457.
|
| [23] |
Lin P, Yu J Z. Generation of reactive oxygen species mediated by humic-like substances in atmospheric aerosols[J]. Environmental Science & Technology, 2011,45(24):10362-10368.
|
| [24] |
Ma Y, Cheng Y, Qiu X, et al. Sources and oxidative potential of water-soluble humic-like substances (HULIS WS) in fine particulate matter (PM2.5) in Beijing[J]. Atmospheric Chemistry and Physics, 2018,18(8):5607-5617.
|
| [25] |
Huang W, Zhang Y, Zhang Y, et al. Development of an automated sampling-analysis system for simultaneous measurement of reactive oxygen species (ROS) in gas and particle phases:GAC-ROS[J]. Atmospheric environment, 2016,134:18-26.
|
| [26] |
Li Q, Shang J, Zhu T. Physicochemical characteristics and toxic effects of ozone-oxidized black carbon particles[J]. Atmospheric Environment, 2013,81(4):68-75.
|
| [27] |
Liu W, Xu Y, Liu W, et al. Oxidative potential of ambient PM2.5 in the coastal cities of the Bohai Sea, northern China:Seasonal variation and source apportionment.[J]. Environmental Pollution, 2018,236:514-528.
|
| [28] |
Ayres J G, Borm P, Cassee F R, et al. Evaluating the toxicity of airborne particulate matter and nanoparticles by measuring oxidative stress potential-A workshop report and consensus statement[J]. Inhalation Toxicology, 2007,20(1):75.
|
| [29] |
Li Q, Wyatt A, Kamens R M. Oxidant generation and toxicity enhancement of aged-diesel exhaust[J]. Atmospheric Environment, 2009,43(5):1037-1042.
|
| [30] |
Yu S Y, Liu W J, Xu Y S, et al. Characteristics and oxidative potential of atmospheric PM2.5 in Beijing:Source apportionment and seasonal variation[J]. Science of the Total Environment, 2019,650:277-287.
|
| [31] |
Charrier J G, Anastasio C. On dithiothreitol (DTT) as a measure of oxidative potential for ambient particles:evidence for the importance of soluble transition metals[J]. Atmospheric Chemistry and Physics, 2012,12(19):9321-9333.
|
| [32] |
Wu C, Wu D, Yu J Z. Quantifying black carbon light absorption enhancement with a novel statistical approach[J]. Atmospheric Chemistry and Physics, 2018,18(1):289-309.
|
| [33] |
Miljevic B, Hedayat F, Stevanovic S, et al. To sonicate or not to sonicate PM filters:reactive oxygen species generation upon ultrasonic irradiation[J]. Aerosol science and technology, 2014,48(12):1276-1284.
|
| [34] |
杨闻达,程鹏,田智林,等.广州市夏秋季HONO污染特征及白天未知源分析[J]. 中国环境科学, 2017,37(6):2029-2039.Yang W D, Cheng P, Tian Z L, et al. Study on HONO pollution characteristics and daytime unknown sources during summer and autumn in Guangzhou, China.[J]. China Environmental Science, 2017, 37(6):2029-2039.
|
| [35] |
Roper C, Chubb L G, Cambal L, et al. Characterization of ambient and extracted PM2.5 collected on filters for toxicology applications[J]. Inhalation Toxicology, 2015,27(13).
|
| [36] |
Yang A, Jedynska A, Hellack B, et al. Measurement of the oxidative potential of PM2.5 and its constituents:The effect of extraction solvent and filter type[J]. Atmospheric Environment, 2014,83:35-42.
|
| [37] |
Choi J H, Kim J S, Kim Y C, et al. Comparative study of PM2.5 and PM10 induced oxidative stress in rat lung epithelial cells.[J]. Journal of Veterinary Science, 2004,5(1):11.
|
| [38] |
Bein K J, Wexler A S. Compositional variance in extracted particulate matter using different filter extraction techniques[J]. Atmospheric Environment, 2015,107:24-34.
|
| [39] |
Hu S, Polidori A, Arhami M, et al. Redox activity and chemical speciation of size fractioned PM in the communities of the Los Angeles-Long Beach harbor[J]. Atmospheric Chemistry and Physics, 2008,8(21):6439-6451.
|
| [40] |
Li N, Sioutas C, Cho A, et al. Ultrafine particulate pollutants induce oxidative stress and mitochondrial damage[J]. Environmental health perspectives, 2003,111(4):455-460.
|
| [41] |
Saffari A, Hasheminassab S, Shafer M M, et al. Nighttime aqueous-phase secondary organic aerosols in Los Angeles and its implication for fine particulate matter composition and oxidative potential[J]. Atmospheric Environment, 2016,133:112-122.
|
| [42] |
Chan J K W, Charrier J G, Kodani S D, et al. Combustion-derived flame generated ultrafine soot generates reactive oxygen species and activates Nrf2antioxidants differently in neonatal and adult rat lungs[J]. Particle and Fibre Toxicology, 2013,10(1):34.
|
| [43] |
Lu Y, Su S, Jin W, et al. Characteristics and cellular effects of ambient particulate matter from Beijing[J]. Environmental Pollution, 2014, 191:63-69.
|
| [44] |
Fujitani Y, Furuyama A, Tanabe K, et al. Comparison of oxidative abilities of PM2.5 collected at traffic and residential sites in Japan. Contribution of transition metals and primary and secondary aerosols[J]. Aerosol and Air Quality Research. 2017,17(2):574-587.
|
|
|
|
