发酵温度对青海农用沼气池微生物群落的影响

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Abstract

In order to investigate the effect of fermentation temperature on the microbial community structure of the rural household biogas digesters in Qinghai Province, twenty-four mud samples from six fermentation temperature periods through the year were collected and analyzed for their changes in microbial community structure using denaturing gradient gel electrophoresis (DGGE). Results showed that fermentation temperature had significant effects on the structure and diversity of microbial communities, and biogas production. Bacteria showed the highest species richness in both the highest (mid-September) and the lowest (mid-March) sampling temperature period, whereas archaea showed the highest species diversity only in mid-September. Firmicutes, Bacteroidetes and Proteobacteria were the most abundant bacterial phyla detected in all samples (total abundance > 72.43%), all of which are important functional bacterial groups in the fermentation system for biogas production. The genera of Petrimas, Clostridium, Tissierella and Pseudomona were the most dominant bacterial groups in all samples, whose total abundance was relatively constant (~32.40%). In archaea, the most dominant group among all samples was the order Methanomicrobiales, with relative abundance of 40.62%~64.74%. The genus Methanogenium was the most dominant species detected for biogas production. Its abundance changed regularly with the fluctuation of fermentation temperature within the range of 32.62%~55.74%, and was consistent with the regularity of biogas production.

Key words： fermentation temperature Qinghai Province rural household biogas digester microbial community structure PCR-DGGE

Received: 30 October 2018

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X172

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	HAN Rui
	ZHU De-rui
	LI Yi
	CHEN Lai-sheng
	ZHANG Guang-nan

Cite this article:

HAN Rui,ZHU De-rui,LI Yi等. The effect of temperature on the microbial community of rural household biogas digesters in Qinghai Province[J]. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(6): 2483-2494.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2019/V39/I6/2483

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