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| Correlation between resistance genes and microbial community in polluted rivers |
| LUO Xiao1, ZHANG Wen-ling2, YUAN Li-xia2, XU Man2, HE Lei2, JIANG Yong-feng2, ZHONG Wei-zhang1, ZHANG Ying2 |
1. School of Environmental Science and Engineering, Pollution Prevention Biotechnology Laboratory of Hebei Province, Hebei University of Science and Technology, Shi jiazhuang 050018, China;
2. School of Civil Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China |
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Abstract The correlation of distribution characteristics, regulations of propagation of tetracyclines and sulfonamide resistance genes (ARGs), and the microbial community structure of the contaminated rivers were studied by using the Miseq sequencing and real-time PCR techniques. The tetracycline resistance genes tetA and tetB and the sulfonamide resistance genes sul1and sul2 were both detected in water and sediment. And the absolute abundances of tetracycline resistance gene and sulfonamide resistance gene were 5.09×103~1.26×107 and 3.94×105~1.32×109copics/L, respectively. The abundances of sul1antibiotic resistance gene was significantly higher than other genes. The main dominant bacteria in the river were Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria and Cyanobacteria. The relative abundance accounted for 95.62% of the total proportion, and there was little difference in overall of the examples. The analysis of resistance gene and microbial community redundancy showed that Methylotenera was the main factor affecting the abundance of tetA resistance gene, Dechloromonas and Clostridium. Sensu stricto 1were the main factors affecting the abundance of tetB resistance gene, and Dechloromonas, Clostridium sensu stricto 1and Methylotenera were the main factors affecting of the abundance of sul1 and sul2 resistance gene distribution.
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Received: 22 November 2018
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