寡养单胞菌对S<sup>2-</sup>氧化特性及主要代谢途径

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Abstract Inorganic sulfur (S^2-) was used as the target pollutant, and a single strain capable of S^2--oxidizing was obtained from Dongsha river (a black-stinking river) located in Beijing. Through both of the physiological-biochemical characteristics and the 16S rRNA sequencing experiment, this single strain was identified as Stenotrophomonas, and named as Stenotrophomonas sp.sp3. The experiment on the growth and S^2--oxidizing characteristics of this strain sp3 was then carried out. The best conditions of S^2--oxidizing were as follows:initial pH of 7.0, 25℃, initial glucose concentration of 0.25% along with initial cell concentration of 1.00g/L. Under the above bio-oxidation conditions, the highest S^2--oxidizing ratio was arrived at 86.6% by this strain sp3 after the bio-reaction of 60h. The concentration of S^2-decreased continually and then kept in a stable status in the whole bio-reaction process, and other sulphur chemical forms including S⁰, S₂O₃^2-, SO₃^2- and SO₄^2- were produced in this period. With the bio-oxidation of S^2-, the concentration of SO₄^2- increased slowly. High-throughput sequencing technologies were used to investigate the main metabolic pathway of S^2-bio-oxidation by this strain sp3. The paracoccus sulfur oxidation process was the possibly main metabolic pathway of S^2-(unstable valence state) to SO₄^2- (stable valence state). It was initially concluded that a portion of S^2-was oxidized to S⁰, while other part of S^2-was directly oxidized to SO₃²; and then S⁰ reacted with SO₃^2- spontaneously to form S₂O₃^2-, while the latter was released SO₃^2- and S⁰ by the disproportionate reaction again. The SO₃^2- accumulated gradually in the system was further directly oxidized to SO₄^2-. This study indicated that this strain sp3 has potential application in water purification of the black-stinking wate bodies.

Key words： S^2- screening sulphur oxidation characteristics metabolic pathway

Received: 03 January 2019

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X172

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	XU Yao-yao
	SONG Chen
	LU Jin-xia
	WANG Jin
	YUE Zheng-bo
	LIU Xiao-ling

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XU Yao-yao,SONG Chen,LU Jin-xia等. S^2--oxidizing characteristics and main bio-oxidation metabolic pathway of Stenotrophomonas sp.sp3[J]. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(8): 3373-3382.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2019/V39/I8/3373

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