|
|
|
| Biofilm formation of ammonia-oxidizing bacteria and anaerobic ammonium-oxidizing bacteria on different biocarries |
| LI Hong-jiang1, WANG Chao2, XU Xiao-chen1, YANG Feng-lin1, ZHANG Shu-shen1 |
1. Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China;
2. Collaborative Innovation Center for Advanced Nuclear Energy Technology, Tsinghua University, Beijing 100084, China |
|
|
|
|
Abstract The typical setbacks of the operation of anaerobic ammonium oxidation (Anammox) process were the serious sludge loss and long startup time. Herein, the biofilm formation experiments were designed to select several commercial biocarriers with better biofilm performance for ammonia-oxidizing bacteria (AOB) and anaerobic ammonium-oxidizing bacteria (AnAOB). The results showed that AQ1polyurethane cubic biocarrier was the most suitable biocarrier for AOB to form biofilm. The ammonia oxidation rate of matured biofilm was (0.81±0.08)mg N/(L·h) and the biofilm biomass was (0.87±0.14)mg VSS. Nitrosomonas was dominant species in the activated sludge. For AnAOB (Candidatus Kuenenia), K3 annular biocarrier (made of polyethylene or polypropylene) was the most suitable biocarrier for the biofilm formation. The anaerobic ammonium oxidation rate of matured biofilm could reach (3.27±0.10)mg N/(L·h), and the biofilm biomass was (2.74±0.40)mg VSS. The biofilm formation of AnAOB on the K3 biocarrier was better than AOB. The reason was that the content of extracellular polymeric substances secreted by AnAOB was higher than that of AOB, and extracellular polymeric substances play an important role in biofilm formation.
|
|
Received: 09 April 2019
|
|
|
|
|
|
| [1] |
Ma B, Wang S, Cao S, et al. Biological nitrogen removal from sewage via anammox:Recent advances[J]. Bioresource Technology, 2016,200:981-990.
|
| [2] |
Delgado Vela J, Stadler LB, Martin KJ, et al. Prospects for biological nitrogen removal from anaerobic effluents during mainstream wastewater treatment[J]. Environmental Science & Technology Letters, 2015,2(9):234-244.
|
| [3] |
Van Hulle S W H, Vandeweyer H J P, Meesschaert B D, et al. Engineering aspects and practical application of autotrophic nitrogen removal from nitrogen rich streams[J]. Chemical Engineering Journal, 2010,162(1):1-20.
|
| [4] |
Sun S, Nàcher CPI, Merkey B, et al. Effective biological nitrogen removal treatment processes for domestic wastewaters with low C/N Ratios:A review[J]. Environmental Engineering Science. 2010,27(2):111-126.
|
| [5] |
Ahn Y. Sustainable nitrogen elimination biotechnologies:A review[J]. Process Biochemistry, 2006,41(8):1709-1721.
|
| [6] |
Zhang Y, Ma H, Chen R, et al. Stoichiometric variation and loading capacity of a high-loading anammox attached film expanded bed (AAEEB) reactor[J]. Bioresource Technology, 2018,253:130-140.
|
| [7] |
陈重军,王建芳,张海芹,等.厌氧氨氧化污水处理工艺及其实际应用研究进展[J]. 生态环境学报, 2014,23(3):521-527. Chen Chongjun, Wang Jianfang, Zhang Haiqin, et al. Research progress in anammox wastewater treatment system and its actual application[J]. Ecology and Environmental Sciences. 2014,23(3):521-527.
|
| [8] |
Zekker I, Rikmann E, Tenno T, et al. Modification of nitrifying biofilm into nitritating one by combination of increased free ammonia concentrations, lowered HRT and dissolved oxygen concentration[J]. J. Environ. Sci. (China), 2011,23(7):1113-1121.
|
| [9] |
何国富,周增炎,高廷耀,等.悬浮填料活性污泥法优化填料投配比试验[J]. 环境工程, 2006,(1):78-80. He Guofu, Zhou Zengyan, Gao Tingyao, et al. Experiment on optimizing the adding ratio of suspended media for suspended media activated-sludge process[J]. Environmental Engineering, 2006,(1):78-80.
|
| [10] |
郑敏,杨波,汪诚文,等.中试MBBR装置强化氨氮去除速率的影响条件研究[J]. 中国环境科学, 2012,32(10):1778-1783. Zheng Min, Yang Bo, Wang Cheng-wen, et al. Enhanced ammonia removal rate in a pilot-scale MBBR[J]. China Environmental Science. 2012,32(10):1778-1783.
|
| [11] |
Gilbert E M, Agrawal S, Karst S M, et al. Low temperature partial nitritation/anammox in a moving bed biofilm reactor treating low strength wastewater[J]. Environmental Science & Technology, 2014, 48(15):8784-8792.
|
| [12] |
周家中,吴迪,韩文杰,等.基于MBBR的CANON工艺处理消化液中式启动[J]. 中国环境科学, 2019,39(6):2378-2386. Zhou Jia-zhong, Wu Di, Han Wen-jie, et al. Pilot scale start-up of CANON disposal sludge-digestion wastewater treatment based on MBBR[J]. China Environmental Science, 2019,39(6):2378-2386.
|
| [13] |
Zekker I, Rikmann, E, Tenno T, et al. Anammox bacteria enrichment and phylogenic analysis in moving bed biofilm reactors[J]. Environmental Engineering Science, 2012,29:946-950.
|
| [14] |
Rouse J D, Burica O, Strazar M, et al. A pilot-plant study of a moving-bed biofilm reactor system using PVA gel as a biocarrier for removals of organic carbon and nitrogen[J]. Water Science & Technology, 2007,55:135-141.
|
| [15] |
Abzazou T, Araujo R M, Auset M, et al. Tracking and quantification of nitrifying bacteria in biofilm and mixed liquor of a partial nitrification MBBR pilot plant using fluorescence in situ hybridization[J]. Science of the Total Environment. 2016,541:1115-1123.
|
| [16] |
Young B, Banihashemi B, Forrest D, et al. Meso and micro-scale response of post carbon removal nitrifying MBBR biofilm across carrier type and loading[J]. Water Research, 2016,91:235-243.
|
| [17] |
Wang X, Xu X, Liu S, et al. Combination of complex adsorption and anammox for nitric oxide removal[J]. J. Hazardous Materials, 2016, 312:175-183.
|
| [18] |
Chen H, Liu S, Yang F, et al. The development of simultaneous partial nitrification, ANAMMOX and denitrification (SNAD) process in a single reactor for nitrogen removal[J]. Bioresource Technology, 2009,100(4):1548-1554.
|
| [19] |
Third KA, Paxman J, Schmid M, et al. Enrichment of Anammox from Activated Sludge and Its Application in the CANON Process[J]. Microbial Ecology, 2005,49(2):236-244.
|
| [20] |
Zhang W, Cao B, Wang D, et al. Influence of wastewater sludge treatment using combined peroxyacetic acid oxidation and inorganic coagulants re-flocculation on characteristics of extracellular polymeric substances (EPS)[J]. Water Research, 2016,88:728-739.
|
| [21] |
Li X Y, Yang S F. Influence of loosely bound extracellular polymeric substances (EPS) on the flocculation, sedimentation and dewaterability of activated sludge[J]. Water Research, 2007,41(5):1022-1030.
|
| [22] |
国家环境保护总局.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社, 2002. The State Environmental Protection Administration. Water and wastewater monitoring and analysis method (Fourth Edition)[M]. Beijing:China Environmental Science Press, 2002.
|
| [23] |
Flemming H, Wingender J. The biofilm matrix[J]. Nature Reviews Microbiology, 2010,8(9):623-633.
|
| [24] |
Guo Y, Liu S, Tang X, et al. Insight into c-di-GMP Regulation in Anammox Aggregation in Response to Alternating Feed Loadings[J]. Environmental Science & Technology, 2017,51(16):9155-9164.
|
| [25] |
孙洪伟,尤永军,白宝霞,等.游离氨对硝化菌活性的短期抑制影响试验研究[J]. 中国给水排水, 2015,31(3):19-23. Sun Hong-wei, You Yong-jun, Bai Bao-xia, et al. Study on short-term inhibition of free ammonia on activity of nitrifying bacteria[J]. China Water & Wastewater, 2015,31(3):19-23.
|
| [26] |
Suneethi S, Joseph K. ANAMMOX process start up and stabilization with an anaerobic seed in Anaerobic Membrane Bioreactor (AnMBR)[J]. Bioresource Technology, 2011,102(19):8860-8867.
|
| [27] |
Wang C, Liu S, Xu X, et al. Potential coupling effects of ammonia-oxidizing and anaerobic ammonium-oxidizing bacteria on completely autotrophic nitrogen removal over nitrite biofilm formation induced by the second messenger cyclic diguanylate[J]. Applied Microbiology and Biotechnology. 2017,101(9):3821-3828.
|
| [28] |
袁冬琴,王毅力.活性污泥胞外聚合物(EPS)的分层组分及其理化性质的变化特征研究[J]. 环境科学, 2012,33(10):3522-3528. Yuan Dong-qin, Wang Yi-li. Study on the stratification components of extracellular polymeric substances (EPS) in activated sludge and their variation characteristics in physicochemical properties[J]. Environmental Science, 2012,33(10):3522-3528.
|
| [29] |
Wett B. Development and implementation of a robust deammonification process[J]. Water Science and Technology, 2007, 56(7):81-88.
|
|
|
|
