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| Effect of the ratios of sodium acetate to sodium propionate on denitrifying phosphorus removal characteristics in the A2/O-BCO process |
| YU Meng1, PAN Ting1, ZHANG Miao1, WANG Yi-xin1, PANG Jing-jin2, JIANG You-fa3, WANG Bao-lin3, HE Cheng-da1 |
1. College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, China;
2. Yangzhou Jieyuan Drainage Company Limited, Yangzhou 225002, China;
3. Jiangsu Yangnong Chemical Company Limited, Yangzhou 225009, China |
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Abstract A denitrifying phosphorus removal system combining anaerobic/anoxic/oxic (A2/O) with biological contact oxidation process (BCO) was used to treat synthetic wastewater. By adjusting the ratio of sodium acetate to sodium propionate (sodium acetate:sodium propionate=1:0,2:1,1:1,1:2 and 0:1), the organic matter removal and denitrifying phosphorus removal characteristics of the system were investigated. The results showed that the ratio of sodium acetate to sodium propionate revealed a negligible effect on organic matter and NH4+-N removals, but the influences on organic consumption in the anaerobic stage, total nitrogen removal as well as the phosphorus release and absorption were obvious. The operation parameters of the system need to be further optimized due to the low phosphorus removal of 50.3%~56.8%. When the sodium acetate/sodium propionate was 1:1, the organic matter consumption in the anaerobic section was the highest, accounting for 61.2% of the total organic matter input, with the maximum anaerobic phosphorus releaseamount(23.2mg/L) and anoxic phosphorus uptake rate (71.4%). However, the TN removal increased with the addition of sodium propionate. High-throughput sequencing results showed that the microbial diversity in the A2/O reactor was lower, while the mixed carbon source sludge was more abundant than that of the single carbon source. Particularly, the amount of Chloroflexi and Saccharibacteria reduced while the Proteobacteria and Bacteroidetes increased in the domesticated sludge. The total proportion of nitrifying bacteria in the BCO reactor was 2.1%~31.4%, and the ammonia oxidizing bacteria (AOB) was dominate which favoured the realization of short-cut nitrification.
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Received: 06 April 2019
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| [1] |
Kim d, Kim T S, Ryu H D, et al. Treatment of low carbon-to-nitrogen wastewater using two-stage sequencing batch reactor with independent nitrification[J]. Process Biochemistry, 2008,43(4):406-413.
|
| [2] |
张丹,胡乐.污水处理厂中反硝化除磷工艺的应用[J]. 广州化工, 2010,38(12):207-209. Zhang D, Hu L, Denitrifying dephosphatation process in wastewater treatment[J]. Guangzhou Chemical Industry, 2010,39(12):207-209.
|
| [3] |
Zhang W, Hou F, Peng Y, et al. Optimizing aeration rate in an external nitrification-denitrifying phosphorus removal (ENDPR) system for domestic wastewater treatment[J]. Chemical Engineering Journal, 2014,24(5):342-347.
|
| [4] |
吉芳英,杨勇光,万小军,等.碳源种类对反硝化除磷系统运行状态的影响[J]. 中国给水排水, 2010,26(15):5-9. Ji F Y, Yang Y G, Wan X J, et al. Effects of carbon source types on operation of denitrifying phosphorus removal system[J]. China Water & Wastewater, 2010,26(15):5-9.
|
| [5] |
Begum S, Batista J. Impacts of carbon source addition on denitrification and phosphorus uptake in enhanced biological phosphorus removal systems[J]. Environmental Letters, 2013,48(4):464-473.
|
| [6] |
Begum S, Batista J R, Shepherd W. Impacts of Denitrification on Biological Phosphorus Removal Using Several Carbon Source Types[J]. Proceedings of the Water Environment Federation, 2009,2009(10):5997-6008.
|
| [7] |
康婷婷,王亮,何洋洋,等.亚硝酸型反硝化除磷工艺特性及其应用[J]. 中国环境科学, 2016,36(6):1705-1714. Kang T T, Wang L, He Y Y, et al. The performance of DPAO using nitrite as electron acceptor and its practical application[J]. China Environmental Science, 2016,36(6):1705-1714.
|
| [8] |
Isaacs S H, Henze M. Controlled carbon source addition to an alternating nitrification-denitrification wastewater treatment process including biological P removal[J]. Water Research, 1994,29(1):77-89.
|
| [9] |
黄惠珺,王淑莹,王中玮,等.不同碳源类型对活性污泥PHA贮存及转化的影响[J]. 化工学报, 2010,61(6):1510-1515. Huang H J, Wang S Y, Wang Z W, et al. Effect of various types of carbon source on biochemical storage and substrate transformation of activated sludge[J]. CIESC Journal, 2010,61(6):1510-1515.
|
| [10] |
Luo D, Yuan L, Liu L, et al. The mechanism of biological phosphorus removal under Anoxic-aerobic alternation condition with starch as sole carbon source and its biochemical pathway[J]. Biochemical Engineering Journal, 2018,132(15):90-99.
|
| [11] |
李洪静,陈银广,顾国维.丙酸/乙酸对低能耗生物除磷脱氮系统的影响[J]. 中国环境科学, 2008,28(8):673-678. Li H J, Chen Y G, Gu G W. Effect of propionic to acetic acid ratio on biological nitrogen and phosphorus removal with low energy consumption[J]. China Environmental Science, 2008,28(8):673-678.
|
| [12] |
Wong P Y, Cheng K Y, Krishna K C B, et al. Improvement of carbon usage for phosphorus recovery in EBPR-r and the shift in microbial community[J]. J. Environmental Management, 2018,218:569-578.
|
| [13] |
国家环境保护局.水和废水监测分析方法[M]. 北京:中国环境科学出版社, 2002. National Environmental Protection Agency. Methods for monitoring and analyzing water and wastewater[M]. Bei Jing:China Environmental Science Press, 2002.
|
| [14] |
Lee J K, Choi C K, Lee K H, et al. Mass balance of nitrogen, and estimates of COD, nitrogen and phosphorus used in microbial synthesis as a function of sludge retention time in a sequencing batch reactor system[J]. Bioresource Technology, 2008,99(16):7788-7796.
|
| [15] |
王聪,王淑莹,张淼,等.厌氧/缺氧/好氧生物接触氧化处理低碳氮比污水的物料平衡[J]. 农业工程学报, 2014,30(19):273-281. Wang C, Wang S Y, Zhang M, et al. Material balance of anaerobic-poor oxygen-aerobiotic biological contact oxidation process treating sewage with low carbon/nitrogen ratio[J]. Transactions of the CSAE, 2014,30(19):273-281.
|
| [16] |
Chuang S, Ouyang C. The biomass fractions of heterotrophs and phosphate-accumulating organisms in a nitrogen and phosphorus removal system[J]. Water Research, 2000,34(8):2283-2290.
|
| [17] |
孙雅雯,张建华,彭永臻,等.外加碳源类型对A2/O-BCO系统脱氮除磷性能的影响[J]. 化工学报, 2018,69(8):3626-3634. Sun Y W, Zhang J H, Peng Y Z, et al. Effect of additional carbon sources on nitrogen and phosphorus removal characteristics in A2/O-BCO process[J]. CIESC Journal, 2018,2018,69(8):3626-3634.
|
| [18] |
李洪静,陈银广,顾国维.丙酸的加入对厌氧-低氧同时生物除磷脱氮系统的影响[J]. 环境科学, 2007,28(8):1681-1686. Li H J, Chen Y G, Gu G W. Addition of propionic acid on simultaneous biological phosphorus and nitrogen removal in anaerobic-low dissolved oxygen system[J]. Environmental Science, 2007,28(8):1681-1686.
|
| [19] |
Chen Y, Randall A A, Mccue T. The efficiency of enhanced biological 995phosphorus removal from real wastewater affected by different ratios of acetic to propionic acid.[J]. Water Research, 2004,38(1):27-36.
|
| [20] |
张淼,张颖,黄棚兰,等.A2/O-MBBR反硝化除磷工艺中有机物的迁移转化及利用[J]. 中国环境科学, 2017,37(11):4132-4139. Zhang M, Zhang Y, Huang P L, et al. Migration and transformation and utilization of organic matter in A2/O-MBBR denitrifying phosphorus removal process[J]. China Environmental Science, 2017, 37(11):4132-4139.
|
| [21] |
Chen Y, Peng C, Wang J, et al. Effect of nitrate recycling ratio on simultaneous biological nutrient removal in a novel anaerobic/anoxic/oxic (A2/O)-biological aerated filter (BAF) system[J]. Bioresource Technology, 2011,102(10):5722-5727.
|
| [22] |
赵青.移动床生物膜多级A/O工艺净化效能与污泥性能研究[D]. 重庆:重庆大学, 2013. Zhao Q. The Purification efficiency and Sludge characteristics of the Moving-bed Biofilm Multi-stage A/O Process[D]. Chong Qing:Chongqing University, 2013.
|
| [23] |
南彦斌,彭永臻,曾立云,等.分段进水对改良A2/O-BAF双污泥系统反硝化除磷脱氮的影响[J]. 环境科学, 2018,39(4):1704-1712. Nan Y B, Peng Y Z, Zeng L Y, et al. Effect of step feed on denitrifying phosphorus and nitrate removal in a modification of the two sludge A2/O-BAF system[J]. Environmental Science, 2018,39(04):1704-1712.
|
| [24] |
Victor Lo K, Tunile I, Tan H, et al. Microwave enhanced advanced oxidation treatment of sewage sludge from the membrane-enhanced biological phosphorus removal process[J]. Separation and Purification Technology, 2018,197(31):202-209.
|
| [25] |
Oehmen A, Saundersa A M, et al. Competition between polyphosphate and glycogen accumulating organisms in enhanced biological phosphorus removal systems with acetate and propionate as carbon sources[J]. Journal of Biotechnology, 2006,123(1):22-32.
|
| [26] |
CECH J. S, Hartman P. Competition Between Polyphosphate and Polysaccharide Accumulating Bacteria in Enhanced Biological Phosphate Removal System[J]. Water Research, 1993,27(7):1219-1225.
|
| [27] |
Burrell P C, Keller J, Blackall L L. Microbiology of a nitrite-oxidizing bioreactor.[J]. Applied Environmental Microbiology, 1998,64(5):1878-1883.
|
| [28] |
吕亮,尤雯,张敏,等.硝化液回流比对ABR-MBR工艺反硝化除磷效能的影响[J]. 环境科学, 2018,39(3):1309-1315. Lv L, You W, Zhang M, et al. Effect of NOx——N Recycling Ratio on Denitrifying Phosphorus Removal Efficiency in the ABR-MBR Combined Process[J]. Environmental Science, 2018,39(3):1309-1315.
|
| [29] |
殷芳芳,王淑莹,昂雪野,等.碳源类型对低温条件下生物反硝化的影响[J]. 环境科学, 2009,30(1):108-113. Yin F F, Wang S Y, Ang X Y, et al. Effects of Carbon Source Types on Denitrification Performance at Low Temperature[J]. Environmental Science, 2009,30(1):108-113.
|
| [30] |
潘芳,郭刚,王鸿,等.缺氧反应时间对反硝化除磷系统脱氮除磷效果的影响[J]. 四川环境, 2014,33(1):36-40. Pan F, Guo G, Wang H, et al. Impact of Anoxic Reaction Time on Denitrifying Phosphorus Removal Performance[J]. Sichuan Environment, 2014,33(1):36-40.
|
| [31] |
杨小梅,张月,潘丹华,等.SRT对A2/O-MBBR工艺中聚磷菌特性的影响[J]. 安全与环境学报, 2017,17(1):256-261. Yang X M, Zhang Y, Pan D H, et al. On the effect of sludge age on the behaviors of phosphorus accumulating organisms in processing A2/O-MBBR[J]. Journal of Safety and Environment, 2017,17(1):256-261.
|
| [32] |
侯爱月,李军,卞伟,等.不同短程硝化系统中微生物群落结构的对比分析[J]. 中国环境科学, 2016,36(2):428-436. Hou A Y, Li J, Bian W, et al. Analysis of microbial community structure in different partial nitrification system[J]. China Environmental Science, 36(2):428-436.
|
| [33] |
Snaidr J, Amann R, Huber I, et al. Phylogenetic analysis and in situ identification of bacteria in activated sludge[J]. Appl Environ Microbiol, 1997,63(7):2884-2896.
|
| [34] |
Cai W, Huang W, Li H, et al. Acetate favors more phosphorus accumulation into aerobic granular sludge than propionate during the treatment of synthetic fermentation liquor[J]. Bioresource Technology, 2016,214:596-603.
|
| [35] |
卞伟,李军,赵白航,等.硝化污泥中AOB/NOB对硝化特性的影响[J]. 中国环境科学, 2016,(8):2395-2401. Bian W, Li J, Zhao B H, et al. The effect of AOB/NOB in nitrifying sludge on nitrification characteristics[J]. China Environmental Science, 2016,(8):2395-2401.
|
|
|
|
