异养硝化-好氧反硝化菌富集驯化过程中微生物种群演变特征——典型城市景观水系

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Abstract To explore the effects of different selective pressures on bacterial community structure during enrichment and domestication of heterotrophic nitrification-aerobic denitrification bacteria, bioinformatics analysis of samples taken from enrichment and domestication systems were carried out, using Miseq high-throughput sequencing. In detail, α-and β-diversity were examined, and network analysis was conducted. Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Actinobacteria, Cyanobacteria, and Acidobacteria were the main phyla identified. Meanwhile, the N-functional bacteria had an increased process. PCA (principal component analysis), NMDS (non-metric multidimensional scaling analysis) and PCoA (principal co-ordinates analysis) showed that microbial community structure was significantly altered with change in temperature, while the influence of different media was small. Network analysis indicated that module hubs and network hubs of bacterial communities were both rare taxa. VIF (variance inflation factor) and RDA (redundancy analysis) showed temperature, ammonia and nitrate were the most important factors affecting bacterial community function and composition. All results together indicate that Miseq high-throughput sequencing was an effective tool to explore changes in bacterial community structure during enrichment and domestication of heterotrophic nitrification-aerobic denitrification bacteria, which could in the future supply a reference to isolate "directional-accurate-efficient" microbial agents.

Key words： heterotrophic nitrification-aerobic denitrification landscape water Miseq sequencing bioinformatics analysis bacterial community structure

Received: 28 April 2019

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X172

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	ZHOU Shi-lei
	ZHANG Yi-ran
	SUN Yue
	YANG Wen-li
	HUANG Ting-lin
	LI Zai-xing
	LUO Xiao
	CUI Jian-sheng
	ZHOU Zi-zhen
	LI Yang

Cite this article:

ZHOU Shi-lei,ZHANG Yi-ran,SUN Yue等. Characteristics of bacterial community structure during the enrichment and domestication of heterotrophic nitrification-aerobic denitrification bacteria based on the typical city landscape water[J]. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(11): 4831-4839.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2019/V39/I11/4831

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