南京市校园室内空气微生物特征

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Abstract

In order to investigate indoor airborne microbial contamination in school classrooms, air samples were collected with six-stage Andersen samplers in classrooms at a kindergarten, a primary school, a middle school and a university in Nanjing, Jiangsu. Ten classrooms in each school were randomly selected. Among the four types of school surveyed, the kindergarten classrooms were found to have the maximum concentration of airborne bacteria and fungi. The mean concentrations of bacteria and fungi were 605CFU/m³ and 648CFU/m³, respectively, which were much higher than that in the other three schools. The size distributions of indoor airborne bacteria and fungi were similar cross all four types of school, with the peak at the stage 5 (1.1~2.1μm). The correlation between indoor environmental parameters and airborne microorganisms was only found to be significant in university classrooms. The daily inhalation doses of airborne bacteria and fungi for kindergarten students were highest at 150.2CFU/kg and 160.9CFU/kg respectively. The study provided some evidence data to evaluate the risk of airborne microbial contamination in schools.

Key words： school airborne microorganism size distribution exposure dose

Received: 17 May 2019

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X51

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	Articles by authors
	SUN Fan
	QIAN Hua
	YE Jin
	ZHUGE Yang
	ZHENG Xiao-hong
	LIAO Wen-hao
	WANG Chong
	WU Xing-tong

Cite this article:

SUN Fan,QIAN Hua,YE Jin等. Characteristics of airborne microorganisms in school classrooms in Nanjing[J]. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(12): 4982-4988.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2019/V39/I12/4982

[1]	World Health Organization. WHO guidelines for indoor air quality:dampness and mould[M]. Denmark, 2009:1-2.
[2]	Mitchell C S, Zhang J F, Sigsgaard T, et al. Current state of the science:health effects and indoor environmental quality[J]. Environmental Health Perspectives, 2007,115(6):958-964.
[3]	Cavaleiro R J, Madureira J, Paciência I, et al. Indoor fungal diversity in primary schools may differently influence allergic sensitization and asthma in children[J]. Pediatric Allergy and Immunology, 2017,28(4):332-339.
[4]	Dannemiller K C, Gent J F, Leaderer B P, et al. Influence of housing characteristics on bacterial and fungal communities in homes of asthmatic children[J]. Indoor Air, 2016,26(2):179-192.
[5]	秦惠,张怡,周斌,等.医院环境致病气溶胶感染风险及其测量方法综述[J]. 暖通空调, 2017,47(5):64-71. Qin H, Zhang Y, Zhou B, et al. Infection risk and measurement of pathogenic aerosol in hospital environment:A review[J]. Heating Ventilating & Air Conditioning, 2017,47(5):64-71.
[6]	Ghosh B, Lal H, Srivastava A. Review of bioaerosols in indoor environment with special reference to sampling, analysis and control mechanisms[J]. Environment International, 2015,85:254-272.
[7]	Thomas R J, Webber D, Sellors W, et al. Characterization and deposition of respirable large-and small-particle bioaerosols[J]. Applied & Environmental Microbiology, 2008,74(20):6437-6443.
[8]	姚文冲,楼秀芹,方治国,等.南方典型旅游城市空气微生物粒径分布特征[J]. 中国环境科学, 2016,36(10):2938-2943. Yao W C, Lou X Q, Fang Z G, et al. The size distribution of airborne microbes in typical tourist city in southeast China[J]. China Environmental Science, 2016,36(10):2938-2943.
[9]	周鑫.颗粒物在人体呼吸系统中传输与沉积的数值模拟研究[D]. 长沙:中南大学, 2010. Zhou X. Numerical investigation on the transport and deposition of particulate matter in human pulmonary airways[D]. Changsha:Central South University, 2010.
[10]	张铭健,曹国庆,冯昕.室内微生物污染水平预测关键技术研究综述[J]. 中国环境科学, 2018,38(11):42-51. Zhang M J, Cao G Q, Feng X. Review of key technologies for forecast of indoor microbial contamination levels[J]. China Environmental Science, 2018,38(11):42-51.
[11]	Prussin A J, Marr L C. Sources of airborne microorganisms in the built environment[J]. Microbiome, 2015,3(1):78.
[12]	张益昭,于玺华,曹国庆,等.生物安全实验室气流组织形式的实验研究[J]. 暖通空调, 2006,36(11):1-7. Zhang Y Z, Yu X H, Cao G Q, et al. Experimental study on air distribution modes in biosafety laboratories[J]. Heating Ventilating & Air Conditioning, 2006,36(11):1-7.
[13]	郑云昊,李菁,陈灏轩,等.生物气溶胶的昨天、今天和明天[J]. 科学通报, 2018,63(10):878-894. Zheng Y H, Li J, Chen H X, et al. Bioaerosol research:yesterday, today and tomorrow (in Chinese)[J]. Chinese Science Bulletin, 2018, 63(10):878-894.
[14]	Pasanen P, Pasanen A L, Jantunen M. Water condensation promotes fungal growth in ventilation ducts[J]. Indoor Air-international Journal of Indoor Air Quality and Climate, 1993,3(2):106-112.
[15]	Stetzenbach L D, Buttner M P, Cruz P. Detection and enumeration of airborne biocontaminants[J]. Current Opinion in Biotechnology, 2004, 15(3):170-174.
[16]	Madureira J, Aguiar L, Pereira C, et al. Indoor exposure to bioaerosol particles:levels and implications for inhalation dose rates in schoolchildren[J]. Air Quality, Atmosphere & Health, 2018,11(8):955-964.
[17]	肖新云,赵先平,王永华,等.空气中微生物的分布规律研究[J]. 中国微生态学杂志, 2015,27(4):406-408. Xiao X Y, Zhao X P, Wang Y H, et al. The characteristics of the distribution of microbes in the air[J]. Chinese Journal of Microecology, 2015,27(4):406-408.
[18]	Salonen H, Duchaine C, Mazaheri M, et al. Airborne culturable fungi in naturally ventilated primary school environments in a subtropical climate[J]. Atmospheric Environment, 2015,106:412-418.
[19]	Hussin N H M, Sann L M, Shamsudin M N, et al. Characterization of bacteria and fungi bioaerosol in the indoor air of selectedprimary schools in Malaysia[J]. Indoor and Built Environment, 2011,20(6):607-617.
[20]	Hwang S H, Kim I S, Park W M. Concentrations of PM10 and airborne bacteria in daycare centers in Seoul relative to indoor environmental factors and daycare center characteristics[J]. Air Quality, Atmosphere & Health, 2017,10(2):139-145.
[21]	Andersen A. New sampler for the collection, sizing and enumeration of viable airborne particles[J]. Journal of Bacteriology, 1958,76(5):471-484.
[22]	齐红霞.病房空调环境微生物菌落特性分析与风险评价[D]. 重庆:重庆大学, 2013. Qi H X. The analysis of the ward air conditioning environmental microbial colony characteristics and risk assessment[D]. Chongqing:Chongqing University, 2013.
[23]	Br?goszewska E, Mainka A, Pastuszka J S, et al. Assessment of bacterial aerosol in a preschool, primary school and high school in Poland[J]. Atmosphere, 2018,9(3):87.
[24]	Environmental Protection Agency. Exposure factors handbook[M]. USA, 2011.
[25]	Su C X, Lau J, Yu F. A case study of upper-room UVGI in densely-occupied elementary classrooms by real-time fluorescent bioaerosol measurements[J]. International Journal of Environmental Research and Public Health, 2017,14(1):51.
[26]	Lee S, Kang S, Bivila C P, et al. Removal of viable airborne fungi from indoor environments by benzalkonium chloride-based aerosol disinfectants[J]. Human and Ecological Risk Assessment:An International Journal, 2015,21(8):2174-2191.
[27]	Heo K J, Lim C E, Kim H B, et al. Effects of human activities on concentrations of culturable bioaerosols in indoor air environments[J]. Journal of Aerosol Science, 2017,104:58-65.
[28]	Johnson D, Lynch R, Marshall C, et al. Aerosol generation by modern flush toilets[J]. Aerosol Science and Technology, 2013,47(9):1047-1057.
[29]	张锐,凌瑜双,陈奕文,等.重庆市地铁车厢空气微生物污染状况调查[J]. 环境与健康杂志, 2015,32(11):1000-1002. Zhang R, Ling Y S, Chen Y W, et al. Investigation of air microorganism pollution in metro carriages in Chongqing[J]. Journal of Environment and Health, 2015,32(11):1000-1002.
[30]	方治国,欧阳志云,刘芃,等.城市居家环境空气细菌群落结构特征[J]. 中国环境科学, 2014,34(10):2669-2675. Fang Z G, Ouyang Z Y, Liu P, et al. Airborne bacterial community composition in family homes in Beijing[J]. China Environmental Science, 2014,34(10):2669-2675.
[31]	郑芷青,谢小保,欧阳友生,等.珠江三角洲城市群大气微生物与环境相关性[J]. 生态环境, 2008,17(6):2304-2311. Zheng Z Q, Xie X B, Ouyang Y S, et al. The relativity between airborne microbes and environmental factors in Pearl River delta' urban agglomeration, Guangdong[J]. Ecology and Environment, 2008, 17(6):2304-2311.
[32]	韩晨,谢绵测,祁建华,等.青岛市不同空气质量下可培养生物气溶胶分布特征及影响因素[J]. 环境科学研究, 2016,29(9):1264-1271. Han C, Xie M C, Qi J H, et al. Size distribution characteristics of culturable bioaerosols in relation to air quality levels in Qingdao[J]. Research of Environmental Sciences, 2016,29(9):1264-1271.
[33]	路瑞,李婉欣,宋颖,等.西安市不同天气下可培养微生物气溶胶浓度变化特征[J]. 环境科学研究, 2017,30(7):1012-1019. Lu R, Li W X, Song Y, et al. Characteristics of culturable bioaerosols in various weather in Xi'an City, China[J]. Research of Environmental Sciences, 2017,30(7):1012-1019.
[34]	王伟,付红蕾,王廷路,等.西安市秋季灰霾天气微生物气溶胶的特性研究[J]. 环境科学学报, 2016,36(1):279-288. Wang W, Fu H L, Wang T L, et al. Characteristics of microbial aerosols on haze days in Autumn in Xi'an, China[J]. Acta Scientiae Circumstantiae, 2016,36(1):279-288.
[35]	方治国,黄闯,楼秀芹,等.南方典型旅游城市空气微生物特征研究[J]. 中国环境科学, 2017,37(8):2840-2847. Fang Z G, Huang C, Lou X Q, et al. Characteristics of airborne microbes in typical tourist city in southeast China[J]. China Environmental Science, 2017,37(8):2840-2847.
[36]	Li Y P, Lu R, Li W X, et al. Concentrations and size distributions of viable bioaerosols under various weather conditions in a typical semi-arid city of Northwest China[J]. Journal of Aerosol Science, 2017,106:83-92.
[37]	杨生玉,王刚,沈永红.微生物生理学[M]. 北京:化学工业出版社, 2007:256-258. Yang S Y, Wang G, Shen Y H. Microbial physiology[M]. Beijing:Chemical Industry Press, 2007:256-258.
[38]	严汉彬,丁力行.控制空调系统微生物污染的温湿度条件分析[J]. 制冷与空调, 2011,11(2):14-17. Yan H B, Ding L X. Analysis of conditions of temperature and humidity to control microbial contaminant in air-conditioning system[J]. Refrigeration and Air-Conditioning, 2011,11(2):14-17.
[39]	Br?goszewska E, Mainka A, Pastuszka J. Bacterial and fungal aerosols in rural nursery schools in southern Poland[J]. Atmosphere, 2016, 7(11):142.
[40]	Fonseca J, Slezakova K, Morais S, et al. Assessment of ultrafine particles in Portuguese preschools:levels and exposure doses[J]. Indoor Air, 2014,24(6):618-628.