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| Reduction of ARB and ARGs by ultraviolet, ozone and combined disinfection technology |
| LI Shu-ming1, WANG Jin1, WANG Hai-chao1, LI Jin-zhao2, HUANG Xue1, WANG Xiao-yue1 |
1. Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Civil Engineering, Beijing Jiaotong University, Beijing 100044, China;
2. China Shenhua Overseas Development & Investment Co. Limited, Beijing 100011, China |
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Abstract Four kinds of resistant bacterium Enterococcus, Brevibacillus, Pseudomonas, and Escherichia coli were isolated from the secondary effluent of a sewage treatment plant. The reduction effect of three disinfection methods of UV, O3 and UV/O3 on ARB and ARGs was evaluated by colony counting method and qPCR. The study showed that the three methods had obvious inactivation of ARB, and the effect on inactivation of Gram-positive bacteria was worse than that of Gram-negative bacteria. The reduction of ARGs varied by the means of disinfection and the types of ARGs, and the result indicated that Brevibacillus showed stronger resistance during the reduction process of ARB and ARGs. In addition, combined with photoreactivation experiments and scanning electron microscopy, it was found that the photoreactivation rate of ARB after UV disinfection for 72h was between 6.93% and 11.60%. There was no photoreactivation phenomenon by O3 due to the destruction of cell structure, which might lead intracellular ARGs to be released into the environment. Meanwhile, the ARB damage caused by UV/O3 was also irreversible.
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Received: 10 May 2019
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