Taking the different ages of Pinus massoniana plantation in the central Guizhou as the research object, using the space-time substitution method, 6 plots of farmland (control), forest gap, middle stage forest, near-mature forest, mature forest and over-mature forest were selected. Chosen a representative day and measure microclimate indicators at different times, and the observation indexes of different vertical heights (0.5, 1.0 and 1.5m) in the sites were compared. The results showed that:(1) Except that the wind speed varied with the measured height (1.5m > 1.0m > 0.5m), the difference was extremely significant (P<0.01), and the other observed indicators had no significant difference with the measured height (P>0.05). (2) During the observation period, there were significant differences in microclimates of Pinus massoniana forests in different forest ages (P<0.05), among which light intensity, photosynthetic photon flux density, air temperature, vapor pressure deficit and wind speed changed to farmland > forest gap > masson pine forests; relative humidity changed to masson pine forests > forest gap > farmland. (3) Compared with farmland, masson pine forests reduced light intensity, photosynthetic photon flux density, air temperature, vapor pressure deficit and wind speed by 917.36%, 837.35%, 5.33%, 30.77%, and 193.58%, respectively, increased relative humidity by 6.39%. Masson pine forests had significant effects of blocking sunlight, reducing air temperature, increasing wetness, maintaining humidity, and keeping microclimate stability under the canopy. In the early stage of succession, the effect of resisting sunlight and wind speed was obvious, while in the later stage, the effect of reducing air temperature, increasing and keeping humidity was remarkable. The comfort evaluation showed that in addition to winter, the masson pine forests in Central Guizhou played a good role in forest rehabilitation and recreation.