酸性矿山废水对沉积物真核微生物群落的影响

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Abstract

Analytical and molecular biology methods were integrated together to investigate the physicochemical factors in water and sediments of Hengshi River as well as the diversity of eukaryotic microorganism community in the sediments. Spearman correlation and canonical correspondence analysis (CCA) were further applied to identify the major factors affecting eukaryotic microorganism community. Results of 18S rRNA gene sequencing indicated that Eukaryotic community of Hengshi River was dominated by Fungi (4.51%~86.69%), Chlorophyta (61%~77.36%) and Ciliophora (0.81%~34.91%). Both the abundance and evenness of eukaryotic communities in sediments significantly and gradually increased along the Hengshi River with decreasing contamination, which suggested that composition of eukaryotic microorganism community partly reflected changes in physicochemical conditions. Sulfate and electric conductivity of the sediments were the major abiotic factors altering the eukaryotic communities in the sediments of AMD impacted Hengshi River, which was different from the findings of prokaryotic microbial communities.

Key words： acid mine drainage eukaryotic microorganisms sulfate electric conductivity next-generation sequencing

Received: 24 May 2019

PACS:

X172

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	Articles by authors
	LIU Fan
	ZHANG Xiao-hui
	TANG Song
	WANG Mao
	LIU Hong-ling

Cite this article:

LIU Fan,ZHANG Xiao-hui,TANG Song等. Effects of acid mine drainage on eukaryotic community in river sediments[J]. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(12): 5285-5292.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2019/V39/I12/5285

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