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| Enhanced nitrogen removal of constructed wetland under low temperature based on cold resistant ammonia-oxidizing functional consortia |
| WANG Shuo1, HU Zhen1, LIU Zi-jun2 |
1. Shandong Province Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao 266200, China;
2. Hebei Sailhero Environmental Protection High-tech Co., Ltd, Shijiazhuang 050000, China |
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Abstract Aiming to solve the problems of decreased nitrogen removal efficiency of constructed wetland (CW) under the low temperature in winter, immobilization of the enriched cold resistant ammonia-oxidizing functional consortia was added into CW to explore its biological enhancement effect on nitrogen removal of CW under low temperature, as well as its microbial function mechanisms. Results showed that, at 5℃, the average ammonia removal rate of CW added with cold resistant ammonia-oxidizing functional consortia cultivated under gradient cooling (R1) and gradient cooling coupled selective inhibition (R2) were 84% and 88%, which were 24% and 28% higher than that in the control, respectively. High-throughput sequencing results revealed that Nitrospira sp., which belongs to complete ammonia oxidizing bacteria, was the dominant bacteria with the relative abundance up to 19.2%, and played the major role in the ammonia oxidation in CW. Analysis of microbial relative expression level showed that the expression abundance of ammonia oxidation gene amoA in the two groups of bio-enhanced constructed wetlands was 2.82×108 and 8.22×108copies/g matrix, which was significantly higher than that in the control, which was 2.50×107copies/g matrix. Generally, the nitrogen removal efficiency of CW could be effectively improved by adding cold resistant ammonia-oxidizing functional consortia.
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Received: 28 July 2019
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