|
|
|
| Impact of different pretreatments on ARGs and live microbial communities in excess sludge |
| MAO Qiu-yan1, ZHAO Xu-ning1, SU Yu-ao1, ZHANG Hui-min1, LIU He1,2,3, FU Bo1,2,3, ZHANG Yan1,2,3 |
1. School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China;
2. Jiangsu Key Laboratory of Anaerobic Biotechnology, Wuxi 214122, China;
3. Jiangsu Collaborative Innovation Center of Water Treatment Technology and Material, Suzhou 215009, China |
|
|
|
|
Abstract Pretreatment can not only facilitate the subsequent anaerobic digestion (AD) of excess sludge, but also may decrease the abundance of antibiotic resistance genes (ARGs) and reduce the risk of ARGs spreading during AD. In this study, the impact of different pretreatments on ARGs and live microbial communities in excess sludge was investigated based on propidium monoazide (PMA) treatment. The results showed that the removal of ARGs by thermo-alkaline, thermal hydrolysis, and microwave pretreatments were 3.32logs, 3.13logs, and 2.95logs, respectviely. The ultrasonic wave pretreatment only removed 0.58logs of the ARGs. With the time extension of thermal hydrolysis, the microorganisms was dramatically removed during 1~2hours. The removal of ARGs was 2.42 logs with thermal hydrolysis extended to 4 hours. Proteobacteria was the dominant bacteria in the raw excess sludge. With the pretreatment of microwave and thermal hydrolysis, Firmicutes and Chloroflexi was greatly improved. The correlation analysis showed that sulI and tetC was very similar that significant positive correlated with several families of microorganisms, which indicated various kinds of the potential host bacteria. However, the good removal of sulI and tetC during pretreatment indicated the poor tolerance of their hosts to the pretreatments. No significant positive correlation was found between ermB and microbial communities, and only few families correlated with tetA. Most ARGs carrying bacteria have little resistance with thermo-alkaline, thermal hydrolysis, and microwave pretreatments, and it is necessary to extend the pretreatment time of thermal hydrolysis appropriately to effectively reduce the ARGs in sludge.
|
|
Received: 26 November 2019
|
|
|
|
|
|
| [1] |
Leclercq S O, Wang C, Sui Z, et al. A multiplayer game: species of Clostridium, Acinetobacter, and Pseudomonas are responsible for the persistence of antibiotic resistance genes in manure‐treated soils [J].Environmental Microbiology, 2016,18(10):3494-3508.
|
| [2] |
Devarajan N, Laffite A, Graham N D, et al.Accumulation of clinically relevant antibiotic-resistance genes, bacterial load, and metals in freshwater lake sediments in central Europe [J].Environmental Science & Technology, 2015,49(11):6528-6537.
|
| [3] |
Aydin S, Ince B, Ince O.Development of antibiotic resistance genes in microbial communities during long-term operation of anaerobic reactors in the treatment of pharmaceutical wastewater [J].Water Research, 2015,83:337-344.
|
| [4] |
Elisabet M, Juan J, Luis B J, et al. Prevalence of antibiotic resistance genes and bacterial community composition in a river influenced by a wastewater treatment Plant [J].PLoS ONE, 2013,8(10):e78906.
|
| [5] |
罗晓,张文丽,袁立霞,等.纳污河流抗性基因和微生物群落相关性[J].中国环境科学, 2019,39(6):2606-2613. Luo X, Zhang W L, Yuan L X, et al.Correlation between resistance genes and microbial community in polluted rivers [J].China Envrionmental Science, 2019,39(6):2606-2613.
|
| [6] |
Luo Y, Mao D Q, Michal R, et al.Trends in antibiotic resistance genes occurrence in the Haihe River, China [J].Environmental Science & Technology, 2010,44(19):7220-7225.
|
| [7] |
杨士林.城市污水处理厂污泥处置技术及利用[J].山东工业技术, 2016,(12):28-28. Yang S L.Sludge disposal technology and utilization in municipal sewage treatment plant [J].Shandong industrial technolog, 2016,(12): 28-28.
|
| [8] |
曹秀芹,陈爱宁,甘一萍,等.污泥厌氧消化技术的研究与进展[J].环境工程, 2008,26(SI):215-219. Cao X, Chen A, Gan Y, et al.New development of anaerobic digestion technology of Sewage Sludge [J].Environmental Engineering, 2008,26(SI):215-219.
|
| [9] |
钱燕云,徐莉柯,苏超,等.初始pH对厌氧环境下污泥中抗生素抗性基因行为特征的影响[J].生态毒理学报, 2015,10(5):47-55. Qian Y Y, Xu L K, Su C, et al.Effect of initial pH on antibiotic resistance genes behavior during anaerobic treatment of sludge [J].Asian Journal of Ecotoxicology, 2015,10(5):47-55.
|
| [10] |
郭思宇,彭永臻,李夕耀,等.热处理对剩余污泥中温厌氧消化的影响[J].中国环境科学, 2017,37(6):2106-2113. Guo S Y, Peng Y Z, Li X Y, et al.Effect of thermal hydrolysis pretreatment on mesophilic anaerobic digestion of excess sludge [J].China Envrionmental Science, 2017,37(6):2106-2113.
|
| [11] |
Tong J, Tang A, Wang H Y, et al. Microbial community evolution and fate of antibiotic resistance genes during sludge treatment in two full-scale anaerobic digestion plants with thermal hydrolysis pretreatment [J].Bioresource Technology, 2019,272:489-500.
|
| [12] |
Rani R U, Kumar S A, Kaliappan S, et al. Impacts of microwave pretreatments on the semi-continuous anaerobic digestion of dairy waste activated sludge [J].Waste Management, 2013,33(5):1119-1127.
|
| [13] |
Mengli Wang.Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline, thermal hydrolysis and ultrasonic pretreatments [J].Frontiers of Environmental Science & Engineering, 2019,13(3):154-163:.
|
| [14] |
廖足良,冉小珊,刘长青,等.热水解和超声波预处理对污泥厌氧消化效能的影响研究[J].环境工程, 2014,32(6):52-56. Liao Z L, Ran X S, Liu C Q, et al.Effect of thermal hydrolysis and ultrasound pretreatment on performance of anaerobic digestion of sludge [J].Environmental Engineering, 2014,32(6):52-56.
|
| [15] |
Tong J, Liu J, Zheng X, et al. Fate of antibiotic resistance bacteria and genes during enhanced anaerobic digestion of sewage sludge by microwave pretreatment [J].Bioresource Technology, 2016,217(10):37-43.
|
| [16] |
陈银广.用于削减污泥厌氧处理过程中抗性基因释放的方法:中国, CN104628234A [P].2015-05-20. Chen Y G.Method for reducing the release of resistance genes in sludge anaerobic treatment: China, CN104628234A [P].2015-05-20.
|
| [17] |
Zhang Y, Li A, Dai T, et al.Cell-free DNA: a neglected source for antibiotic resistance genes spreading from WWTPs [J].Environmental Science & Technology, 2017,52(1):248-257.
|
| [18] |
Mao D, Luo Y, Mathieu J, et al.Persistence of extracellular DNA in river sediment facilitates antibiotic resistance gene propagation [J].Environmental science & technology, 2013,48(1):71-78.
|
| [19] |
Miller J H, Novak J T, Knocke W R, et al.Survival of antibiotic resistant bacteria and horizontal gene transfer control antibiotic resistance gene content in anaerobic digesters [J].Frontiers in Microbiology, 2016,7:263.
|
| [20] |
Nocker A, Cheung C Y, Camper A K.Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs.dead bacteria by selective removal of DNA from dead cells [J].Journal of Microbiological Methods, 2006,67(2):310-320.
|
| [21] |
Jessica K, Jack T T, Hung L, et al. Molecular pathogen detection in biosolids with a focus on quantitative PCR using propidium monoazide for viable cell enumeration [J].Journal of Microbiological Methods, 2011,87(3):263-272.
|
| [22] |
国家环境保护总局.水和废水监测分析方法[M].北京:中国环境科学出版社, 2002. State Environmental protection administration.Water and wastewater monitoring and analysis methods [M].China Environmental Science Press, 2002.
|
| [23] |
Wang W P.Assay Study on Content of polysaccharides in stanuntonia Chinensis by phenol-sulfuric acid method [J].Food Science, 2007, 28(4):276-278.
|
| [24] |
吕媛,马钰,冯志明,等.二喹啉甲酸法在牛奶蛋白质定量中的应用[J].食品科学, 2010,31(6):151-154. LÜ Y, Ma Y, Feng Z M, et al.Bicinchoninic acid method for determination of protein content in milk [J].Food Science, 2010, 31(6):151-154.
|
| [25] |
Guo F, Zhang T.Detecting the nonviable and heat-tolerant bacteria in activated sludge by minimizing DNA from dead cells [J].Microbial Ecology, 2014,67(4):829-36.
|
| [26] |
Wang H, Chen M J.Construction of a plasmid and a standard curve for real-time quantitative PCR of the cold-induced Cor3gene from volvariella volvacea [J].Acta Edulis Fungi, 2007,(3):20-23.
|
| [27] |
Chen B W, Liang X M, Huang X P, et al. Differentiating anthropogenic impacts on ARGs in the Pearl River Estuary by using suitable gene indicators [J].Water Research, 2013,47(8):2811-2820.
|
| [28] |
Jiang L, Hu X L, Xu T, Zhang H C, et al. Prevalence of antibiotic resistance genes and their relationship with antibiotics in the Huangpu River and the drinking waters sources, Shanghai, China [J].Science of the Total Environment, 2013,458-460C3:267-272.
|
| [29] |
Huerta B, Marti E, Gros M, et al. Exploring the links between antibiotic occurrence, antibiotic resistance, and bacterial communities in water supply reservoirs [J].Science of the Total Environment, 2013,s456-457(7):161-170.
|
| [30] |
Ayodele A, Adeniyi O, Isaac O.Molecular characterization of selected multidrug resistant Pseudomonas from water distribution systems in southwestern Nigeria [J].Annals of Clinical Microbiology and Antimicrobials, 2015,14(1):39.
|
| [31] |
Zhang Y, Chen L J, Sun R H, et al. Ammonia-oxidizing bacteria and archaea in wastewater treatment plant sludge and nearby coastal sediment in an industrial area in China [J].Applied Microbiology and Biotechnology, 2015,99(10):4495-4507.
|
| [32] |
He S, Gall D L, Mcmahon K D.Candidatus accumulibacter" population structure in enhanced biological phosphorus removal sludges as revealed by polyphosphate kinase genes [J].Applied and Environmental Microbiology, 2007,73(18):5865-5874.
|
| [33] |
张心齐.陆地热泉及深海热液沉积物生境中的嗜热菌多样性研究[D].杭州:浙江大学, 2009. Zhang X Q.Diversity of thermophiles in the biotopes of terrestrial hot springs and deep-sea hydrothermal sediment [D].Hangzhou: Zhe Jiang University, 2009.
|
| [34] |
Liang B, Wang L Y, Mbadinga S M, et al. Anaerolineaceae and Methanosaeta turned to be the dominant microorganisms in alkanes-dependent methanogenic culture after long-term of incubation [J].AMB Express, 2015,5(1):37.
|
| [35] |
金涣峻.半透膜覆盖好氧堆肥系统处理牛粪/秸秆的效能及功能微生物作用机制[D].哈尔滨:哈尔滨工业大学, 2017. KIM W J.Efficiency and functional microbial mechanism of aerobic composting system on cattle manure/straw with semipermeable membrane [D].Harbin: Harbin Institute of Technology, 2017.
|
| [36] |
陆萍.安徽部分地区猪丹毒杆菌的分离鉴定及生物学特性研究[J].微生物学通报, 2014,41(9):1822-1828. Lu P.Isolation, identification and characterization of Erysipelothrix rhusiopathiae inAnhui province [J].Institute of Microbiology, 2014, 41(9):1822-1828.
|
|
|
|
