氨氮对餐厨垃圾厌氧消化性能及微生物群落的影响

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Abstract A series of batch experiments were conducted under ammonia-N stress conditions to investigate the effects of ammonium (TAN) on performance and microbial community during the anaerobic digestion (AD) process of food waste. At different TAN levels, the response of AD process parameters and the dynamics of microbial communities were investigated by physico-chemical analysis along with Miseq high-throughput sequencing analysis. Results showed that, with the increase of TAN, the methane recovery rate decreased from (96.53±2.66)% to (63.13±0.73)% while the digestion time increased from 435h to 915h. After continuous acclimation, the gas production performance was completely recovered in the test group with TAN concentration of 3000mg/L. However, the gas production in the group with high ammonia concentration (³6000mg/L) was still inhibited and did not recovered by acclimation. Compared with acetate metabolism, the metabolism of long-chain volatile fatty acids (LCVFAs) was more tolerant to ammonia level (6000mg/L), but difficult to recover by acclimation if inhibited. At the microbial communities, the high abundance of hydrolytic and fermentative bacteria with redundant function ensured adequate hydrolysis and acidification at different TAN concentrations. Ammonia-sensitive Methanosaeta and Methanospirillum could also be replaced by ammonia-resistant Methanosarcina and Methanoculleus via acclimation, and thus ensure methanogenesis. In comparison, acetogenic bacteria featured with highly specialized function, and only the C₄~C₁₈ and propionate degraded Syntrophomonas and Pelotomaculum were detected. Under continuous high ammonia exposure, the abundance of the former bacteria recovered to a certain degree, while the latter could not be acclimatized. Eventually, the AD system was observed to accumulate LCVFAs (mainly propionate), and the gas production process was deteriorated. Therefore, the key contributor to ammonia inhibition turned out to be the syntrophic degradation of LCVFAs.

Key words： food waste anaerobic digestion ammonia inhibition process performance microbial community

Received: 10 January 2020

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	ZHANG Hong
	LI Lei
	PENG Yun
	YANG Ping-jin
	HUANG Qian
	PENG Xu-ya
	WANG Xiao-ming

Cite this article:

ZHANG Hong,LI Lei,PENG Yun等. Effects of ammonia on anaerobic digestion of food waste: Process performance and microbial community[J]. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(8): 3465-3474.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I8/3465

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