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| Effects of different substrate biochar on the performance and microecology of anaerobic biological treating food waste |
| TANG Lin-qin1, SU Cheng-yuan1,2, ZHAO Li-jian1, WANG Peng-fei1, WANG An-liu1, LI Fei-qian1, FAN Cui-ping1, HUANG Xin-yue1 |
1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin 541004, China;
2. School of Environment and Resources, Guangxi Normal University, Guilin 541004, China |
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Abstract The effects of sewage sludge (SS), food waste (FW), corn cob (CC) and bagasse (BG) biochar on the anaerobic biological treatment of food waste were investigated. Meanwhile, the key enzyme activity, microbial community distribution and metabolic pathway of anaerobic sludge were analyzed. The average COD removal rate increased by 29.49%, 23.16%, 29.42%, and 40.32% after addition of the four kinds of biochar in the anaerobic reactor, respectively. The activity of acetokinase in the four anaerobic reactors was 0.40, 0.42, 0.96 and 0.98μmol/g, respectively. The high acetokinase activity of the anaerobic sludge in the CC and BG groups demonstrated that the addition of the CC and BG biochar promoted the hydrolysis and acidification of food waste. In addition, the ratio of protein/polysaccharide of extracellular polymeric substances in the anaerobic sludge was 0.415, 0.56, 1.89 and 2.8, respectively. It was demonstrated that adding CC and BG biochar improved the stability of anaerobic sludge. In the four anaerobic reactors, Bacteroidetes, Proteobacteria and Firmicutes were the dominant phylum, and the addition of BG biochar promoted the growth of Proteobacteria and Firmicutes. As for the archaea, Methanobacterium and Methanothrix were the dominant species. Methanobacterium in the SS group had highest abundance (53.48%), while Methanothrix in the BG group had the highest abundance (42.72%). Kyoto Encyclopedia of Genes and Genomes (KEGG) functional analysis showed that archaea and bacteria were mainly metabolized by carbohydrate and amino acids. In addition, the transport level of microbial membrane was improved in presence of BG and SS biochar.
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Received: 12 March 2020
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