|
|
Effect of light intensity on the growth of Scenedesmus quadricauda at cell scale |
LOU Xiao-fei1, DU Yan-sheng2, ZHANG Hai-ping1 |
1. Department of Environmental Science, Tongji University, Shanghai 200092, China;
2. Tongji Architectural Design(Group) Co., Ltd., Shanghai 200092, China |
|
|
Abstract Achievements have been made on the research in the influence of light on the algal growth. While the mechanism of the influence remains unclear in many aspects. The dynamic growth of Scenedesmus quadricauda cells was observed with the parallel plate flow chamber device and the computer vision technology in the study. The effect of different light intensity on the growth of Scenedesmus quadricauda were studied at a single cell scale. The individual growth curve model of Scenedesmus quadricauda has been established, which fitted the measured data well. The results showed that the largest maximum volumic growth rate of algal cells appeared under 8000lux light intensity, which could be regarded as the optimal light intensity for Scenedesmus quadricauda. The appropriate light conditions could increase algal cell's size before division. The algal cell's size before division increased with the increase of light intensity when it was lower than 8000lux. While the size decreased with the increase of light intensity when it was higher than 8000lux. In addition, higher light intensity was advantageous for an algal cell to adapt to the new environment and to reduce the time required for the algae recruitment.
|
Received: 23 March 2020
|
|
|
|
|
[1] |
Weinstein B G. A computer vision for animal ecology[J]. Journal of Animal Ecology, 2018,87(3):533-545.
|
[2] |
Hedrick T L. Software techniques for two-and three-dimensional kinematic measurements of biological and biomimetic systems[J]. Bioinspiration & Biomimetics, 2008,3(3):034001.
|
[3] |
Samantaray A, Yang B, Dietz J E, et al. Algae detection using computer vision and deep learning[J]. arXiv Preprint arXiv:2018, 1811:10847.
|
[4] |
Medina E, Petraglia M R, Gomes J G R C, et al. Comparison of CNN and MLP classifiers for algae detection in underwater pipelines[C]//2017Seventh International Conference on Image Processing Theory, Tools and Applications (IPTA) IEEE, 2017:1-6.
|
[5] |
Penmetcha M, Luo S, Samantaray A, et al. Computer vision-based algae removal planner for Multi-robot Teams[C]//2019IEEE International Conference on Systems, Man and Cybernetics (SMC) IEEE, 2019:1575-1581.
|
[6] |
Medina E, Petraglia M R, Gomes J G R C. Neural-network based algorithm for algae detection in automatic inspection of underwater pipelines[C]//Mexican International Conference on Artificial Intelligence, Springer, Cham., 2016:141-148.
|
[7] |
毕研刚,王鹏,丁天怀.藻细胞浓度的虚拟采样检测方法[J]. 清华大学学报:自然科学版, 2010,50(11):1807-1810. Bi Y G, Wang P, Ding T H. Method for measuring algae cell concentrations via virtual sampling[J]. Journal of Tsinghua University (Science & Technology), 2010,50(11):1807-1810.
|
[8] |
柴小颖.光照和温度对三峡库区典型水华藻类生长的影响研究[D]. 重庆:重庆大学, 2009. Chai X Y. Behavior study on the role of irradiance and temperature on the algae bloom of typical algae in Three-Gorges valley[D]. Chongqing:Chongqing University, 2009.
|
[9] |
高静思,朱佳,董文艺.光照对我国常见藻类的影响机制及其应用[J]. 环境工程, 2019,37(5):114-116. Gao J S, Zhu J, Dong W Y. Influence mechanism of light on common algae and its application[J]. Environmental Engineering, 2019,37(5):114-116.
|
[10] |
孟顺龙,裘丽萍,王菁等.光照对普通小球藻和鱼腥藻生长竞争的影响[J]. 生态环境学报, 2015,24(10):1654-1659. Meng S L, Qiu L P, Wang J, et al. Effect of light intensity on growth and competition between Chlorella Vulgaris and Anabaena[J]. Ecology and Environmental Sciences, 2015,24(10):1654-1659.
|
[11] |
Wojewodzic M W, Kyle M, Elser J J, et al. Joint effect of phosphorus limitation and temperature on alkaline phosphatase activity and somatic growth in Daphnia magna[J]. Oecologia, 2011,165(4):837-846.
|
[12] |
Yeh T Y, Ke T Y, Lin Y L. Algal growth control within natural water purification systems:macrophyte light shading effects[J]. Water, Air, & Soil Pollution, 2011,214(1-4):575-586.
|
[13] |
龙天渝,周鹏瑞,吴磊.环境因子对香溪河春季藻类生长影响的模拟实验[J]. 中国环境科学, 2011,31(2):327-331. Long T Y, Zhou P R, Wu L. The simulating experiment for the impacts of environmental factors on Spring algae growth in Xiangxi River[J]. China Environmental Science, 2011,31(2):327-331.
|
[14] |
Tamburic B, Zemichael F W, Maitland G C, et al. Parameters affecting the growth and hydrogen production of the green alga Chlamydomonas reinhardtii[J]. International Journal of Hydrogen Energy, 2011,36(13):7872-7876.
|
[15] |
唐婉容,王璐.平行平板流动腔的研究进展[J]. 口腔材料器械杂志, 2012,21(2):96-97,103. Tang W R, Wang L. Research progress of the parallel plate flow chamber[J]. Journal of Dental Materials and Devices, 2012,21(2):96-97,103.
|
[16] |
Busscher H J, Van der Mei H C. Microbial adhesion in flow displacement systems[J]. Clinical Microbiology Reviews, 2019,19(1):127-141.
|
[17] |
Gabriel S, Katharina B, Iris H, et al. Microcosm design and evaluation to study stream microbial biofilms[J]. Limnology & Oceanography Methods, 2006,4(11):436-447.
|
[18] |
刘雨佳,丁皓,张迎,等.体外内皮细胞培养装置的研制与实验研究[J]. 医用生物力学, 2019,34(5):541-547. Liu Y J, Ding H, Zhang Y, et al. The development and experimental study of endothelial cell culture device in vitro[J]. Journal of Medical Biomechanics, 2019,34(5):541-547.
|
[19] |
王艳霞.运动引起的血流剪切力信号调控内皮细胞功能的体外研究[D]. 大连:大连理工大学, 2019. Wang Y X. An in vitro study on endothelial cell function modulated by exercise-induced vascular wall shear stress[D]. Dalian:Dalian University of Technology, 2019.
|
[20] |
曾鲁,柔丽.医学细胞生物学[M]. 北京:北京医科大学,中国协和医科大学联合出版社, 1992. Zeng L, Rou L. Medical cell biology[M]. Beijing:Beijing Medical University, Union Medical College Press, 1992.
|
[21] |
Jørgensen S E, Fath B D. Fundamentals of ecological modelling:applications in environmental management and research[M]. Elsevier, 2011.
|
[22] |
Lodish H, Berk A, Kaiser C A, et al. Molecular cell biology[M]. Macmillan, 2008.
|
[23] |
Zhou B, Bi Y H, Hu Z Y. Effects of temperature on the buoyancy of Microcystis aeruginosa[J]. China Environmental Science, 2014,34(7):1847-1854.
|
[24] |
袁著涛,董晓煜,刘升平.温度和光照对单细胞绿藻1102生长影响的研究[J]. 水产科学, 2014,33(2):123-126. Yuan Z T, Dong X Y, Liu S P. Effects of temperature and light on the growth of unicellular Chlorella 1102[J]. Fisheries Science, 2014, 33(2):123-126.
|
[25] |
逯南南,褚福敏.温度及光照强度对藻类生长的影响[C]//第四届"黄河杯"城镇饮用水安全保障技术论坛暨城市供水水质监测技术交流会, 2010:137-140. Tong N N, Chu F M. Effects of temperature and light intensity on algae growth[C]//The 4th "Yellow River Cup" Urban Drinking Water Safety Technology Forum and Urban Water Quality Monitoring Technology Exchange Conference, 2010:137-140.
|
[26] |
任翔宇,王铭玮,顾詠洁,等.光照强度及温度对青草沙水库蓝藻暴发风险的模拟试验[J]. 净水技术, 2012,31(4):55-60. Ren X Y, Wang M W, Gu Y J, et al. Simulation experiment in different light intensity and temperature gradients for risk of blue-green algae outbreak in Qingcaosha reservoir[J]. Water Purification Technology, 2012,31(4):55-60.
|
[27] |
胡小贞,金相灿,储昭升,等.太湖铜绿微囊藻与四尾栅藻的光竞争及模拟优势过程初探[J]. 农业环境科学学报, 2005,(3):538-543. Hu X Z, Jin X C, Chu Z S, et al. Light competition between Microcystis aeruginosa and Scendesmus quadricauda from Taihu Lake and the dominance process simulation in microcosm[J]. Journal of Agro-Environment Science, 2005,(3):538-543.
|
|
|
|