AOB去除炔雌醇的共代谢与硝基化协同作用

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Abstract In this study, Nitrosomonas europaea, one type of ammonia oxidizing bacteria, was used to remove 17α-ethinylestradiol (EE2). The role of ammonia nitrogen in EE2 degradation by AOB and the degradation mechanism of EE2 were investigated. The results showed that degradation of EE2 by N. europaea was a co-metabolic process, and ammonia nitrogen was necessary for co-metabolism of EE2. The NO₂^--N produced during the ammonia oxidation process could nitrate EE2 under acidic condition. The nitration of EE2 obeyed first-order reaction kinetics and the degradation rate constants were positively correlated with the concentration of nitrite, H⁺ and FNA. The biodegradation of EE2 by N. europaea was proved by suppressing nitration of EE2 through controlling pH higher than 7.5. The co-metabolism reaction fitted first-order kinetic model with the degradation rate constant of 0.0069h^-1. Biodegradation and nitration of EE2 were synergistic for EE2 removal when pH was lower than 7.5 during ammonia oxidation process. And EE2 removal by synergistic function obeyed first-order reaction kinetics with the degradation rate constant of 0.0093h^-1. In addition, unreported EE2 biodegradation product M613 was found in this study. Its estrogen effect and toxicity need to be further investigated.

Key words： ammonia-oxidizing bacteria (AOB) 17α-ethinylestradiol (EE2) co-metabolism nitration

Received: 09 May 2020

PACS:	X703
	X172

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	WANG Li-li
	JIANG Xiao-man
	Li An-jie

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WANG Li-li,JIANG Xiao-man,Li An-jie. Synergistic effect of co-metabolism and nitration for the removal of EE2 by AOB[J]. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(12): 5246-5252.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I12/5246

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