同化和共代谢降解氯苯菌株的筛选与特性研究

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Abstract In this study, a novel chlorobenzene (CB)-degrading strain was isolated on the contaminated site and identified as Serratia marcescans TF-1. The assimilation degradation analysis showed that CB can be used by strain TF-1as the sole carbon source and energy under aerobic conditions, and the corresponding average growth rate, the maximum specific growth rate (μ_max), the CB degradation rate, and the maximum CB tolerance concentration were 0.0063~0.022g_cell/(mol_CB·h), 0.015~0.42h^-1, 1.35~4.47mol/(g_cell·h), 200mg/L, respectively. Moreover, sodium succinate and sodium citrate can be co-metabolized by strain TF-1as substrates. μ_max (sodium citrate), μ_max (sodium succinate), V_CB(sodium citrate) and V_CB(sodium succinate) were 0.21~0.87h^‑1, 0.20~0.81h^‑1, 0.15~0.47mol/(g_cell·h) and 0.17~0.48mol/(g_cell·h) respectively, when CB concentration was less than 80mg/L, and μ_max (sodium citrate), μ_max (sodium succinate), V_CB(sodium citrate) and V_CB(sodium succinate) were 0.086~0.21h^‑1, 0.17~0.25h^‑1, 0.61~1.11mol/(g_cell·h) and 0.56~0.95mol/(g_cell·h) respectively, when CB concentration was greater than 80mg/L. The results showed that the substrate type is the key factor in the co-metabolic degradation. Finally, the effects of temperature, pH and inoculation concentration on the degradation of CB by TF-1 were investigated. The results showed that the growth temperature and pH range of TF-1 were 20~35℃ and 5~9, respectively. The optimal growth temperature of 30℃, pH of 7 and inoculation concentration of 5% were also obtained. The results indicated that TF-1 had a wider temperature and pH application range, stronger degradation ability, and higher tolerance to pollutant concentration. In addition, CB could be used directly or co-metabolized by strain TF-1, which was suitable for application in oligotrophic and eutrophic contaminated sites. This study provided effective biological resources for in-situ CB contaminated site remediation.

Key words： assimilation co-metabolism chlorobenzene functional bacteria high tolerance

Received: 16 June 2020

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X172

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	Articles by authors
	GUO Jiang-feng
	XING Zhi-lin
	WANG Yong-qiong
	CAO Kun
	ZHANG Xue-lian
	GOU Fang
	SHI Yun-chun
	LIU Li-sha
	ZHAO Tian-tao

Cite this article:

GUO Jiang-feng,XING Zhi-lin,WANG Yong-qiong等. Screening and characterization of an assimilation and co-metabolism-degrading chlorobenzene bacterial strain[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(2): 902-912.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I2/902

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