武汉东湖沉积物好氧氨氧化微生物时空分布

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Abstract The amoA gene abundances of ammonia-oxidizing archaea (AOA-amoA) and bacteria (AOB-amoA) in the sediments of Lake Donghu, Wuhan, was determined by the real-time quantitative polymerase chain reaction (qPCR). Moreover, the influence of nitrogen content on the temporal and spatial distribution of AOA and AOB was analyzed. The results showed that the abundance of AOA-amoA was higher than that of AOB-amoA, indicating greater contribution of AOA-amoA to the ammonia oxidation process. Meanwhile, the abundances of AOA-amoA and AOB-amoA decreased with elevated sediment depth. In addition, the concentrations of total nitrogen (TN), ammonia nitrogen (NH₄⁺-N), nitrate nitrogen (NO₃^--N) and nitrite nitrogen (NO₂^--N) in interstitial water were 6.28~33.56, 2.71~22.7, 0.12~0.98 and 0.01~0.13mg/L, respectively. The average concentrations of TN, NH₄⁺-N, NO₃^--N and NO₂^--N in overlying water were 1.68, 0.79, 0.16 and 0.04mg/L, respectively, while in surface water were 1.34, 0.62, 0.11 and 0.03mg/L, respectively. These results suggested that the sediments of Lake Donghu were important sources of nutrient. In addition, the abundance of AOA-amoA was positively correlated with the concentrations of NH₄⁺-N and NO₂^--N in interstitial water (P<0.05), while the abundance of AOB-amoA was positively correlated with the concentration of NO₂^--N in interstitial water (P<0.05).

Key words： qPCR sediment ammoxidation archaea ammonia oxidizing bacteria abundance

Received: 24 August 2020

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X524

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	ZHANG Zhi-zhong
	CHENG De-xi
	LIAO Ming-jun
	WANG Shu-lian
	LI Zhu

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ZHANG Zhi-zhong,CHENG De-xi,LIAO Ming-jun等. Spatiotemporal distribution of aerobic ammonia-oxidizing microorganisms in sediments of Lake Donghu, Wuhan[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(4): 1917-1924.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I4/1917

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