磷化氢生物净化体系及抑制作用机理

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Abstract Two electron transport inhibitors (rotenone and anti-mycin A) were introduced to phosphine bio-purification systems, then comparative experiments were conducted to investigate the variations of the microbial growth and metabolisms, phosphorus transport and transformation, the production of reactive oxygen species (ROS), and oxidase activities. When rotenone and anti-mycin A were respectively added to R1 and R2 bioreactors, the average O₂^-· content in these two systems increased to 0.68 and 0.96 mmol/g during the operation time of 14~17 days, which was significantly higher than control group (i.e. R0 reactor). Besides O₂^-·, another kind of reactive oxygen species, i.e. HO·, was also detected in bio-purification systems. In addition, due to the ROS accumulation, the malondialdehyde (MDA) content in microorganisms kept at a high level, caused the purification efficiency of phosphine less than 75%. The average phosphine removal efficiency in the R1 and R2 bioreactors decreased to 65.1% and 59.5% respectively, after 15~17 days operation. The factors, e.g., pH, ROS, and oxidase activity, had significant effects on phosphine biological purification. As metal cations such as Mg²⁺ and Cu²⁺ migrated into the microbial cells, the enzyme activities of superoxide dismutase and catalase were dramatically improved, which led to the alleviation of the oxidative stress derived from the reactive species such as O₂^-· and HO·. As the consequence, the purification efficacy of phosphine was improved moderately.

Key words： phosphine biotransformation inhibition reactive oxygen species

Received: 20 September 2020

PACS:

X701

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	YU Shuo
	LIU Shu-gen
	LI Ting

Cite this article:

YU Shuo,LIU Shu-gen,LI Ting. Inhibition mechanism of phosphine biological purification system[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(5): 2219-2225.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I5/2219

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